›› 2011, Vol. 31 ›› Issue (6): 739-.doi: 10.3969/j.issn.1674-8115.2011.06.013

• Original article (Basic research) • Previous Articles     Next Articles

Effects of BMSCs on expression of CD4+CD25+Treg in rats with unilateral ureter obstruction

ZHUANG Feng1,2, LIU Ying-li2, ZHANG Wei2, LI Ning-li1   

  1. 1.Basic Medical College, Shanghai Jiaotong University, Shanghai Institute for Immunology, Shanghai 200025, China；2.Department of Nephrology, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-06-28 Published:2011-06-27
  • Supported by:

    Shanghai Natural Science Foundation of China, 09ZR1416800；Shanghai Jiaotong University Foundation, YG2010MS83

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Abstract:

Objective To investigate the effects of bone mesenchymal stem cells (BMSCs) on expression of CD4+CD25+ Treg in peripheral blood in rats with unilateral ureter obstruction (UUO). Methods BMSCs were isolated from Wistar rats, and were cultured and passaged in vitro. Cell suspension was prepared after identification of adipocytes differentiated from BMSCs. Fifty-four Wistar rats were randomly divided into sham operation group, BMSCs intervention group and normal saline infusion group (n=18). Obstructive nephropathy models were established in rats of BMSCs intervention group and normal saline infusion group by UUO, and were injected with BMSCs and normal saline respectively through inferior vena cava. Three days, 7 d and 14 d after model establishment, peripheral blood was taken, and renal tissue samples were obtained after sacrifice of rats (n=6 on each day in each group). The percents of CD4+CD25+Treg in CD4+T cells (CD4+CD25+Treg/CD4+Treg) were detected by flow cytometry, the pathological changes of renal tissues were observed with HE staining by light microscopy, and the expression of CD4+CD25+Treg specific marker FoxP3 in renal tissues was detected by immunohistochemical method. Results CD4+CD25+Treg/CD4+Treg in peripheral blood in BMSCs intervention group and normal saline infusion group were significantly higher than that in sham operation group at each time point after model establishment (P<0.05), while there was no significant difference between BMSCs intervention group and normal saline infusion group (P>0.05). Three days and 7 d after model establishment, rats in normal saline infusion group experienced renal interstitial edema, progressive development of renal and renal interstitial lymphocyte and macrophage infiltration, and rats in BMSCs intervention group had better renal tissue structure than those in normal saline infusion group. Three days and 7 d after model establishment, the expression of FoxP3 in renal tissues in BMSCs intervention group was significantly higher than that in sham operation group and normal saline infusion group （P<0.05). Conclusion Renal interstitial fibrosis of early stage can be improved by BMSCs injection in rats with obstructive nephropathy, which may be related to the increase of local expression of FoxP3 and generation of CD4+CD25+Treg.

Key words: bone mesenchymal stem cells, regulatory T cells, renal interstitial fibrosis, unilateral ureter obstruction