›› 2012, Vol. 32 ›› Issue (4): 401-.doi: 10.3969/j.issn.1674-8115.2012.04.006

• Monographic report (Urinary dysfunction and pelvic reconstruction) • Previous Articles     Next Articles

Adhesion behavior of rabbit adipose-derived stromal cells on chitosan-modified polypropylene mesh

CHENG Hui1, WU Qing-kai1, SUN Jun-fen2, ZHANG Rong1, TENG Yin-cheng1, LUO Lai-min1, DOU Hong-jing3   

  1. 1. Department of Obstetrics and Gynecology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；2.College of Materials Science and Engineering, Donghua University, Shanghai 201620, China；3.School of Materials Science and Engineering, Shangahai Jiaotong University, Shanghai 200240, China
  • Online:2012-04-28 Published:2012-04-27
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2010MS47

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Abstract:

Objective To study the technical parameters of chitosan-modified polypropylene mesh, and observe the adhesion behavior of rabbit adipose-derived stromal cells (ADSCs) on the modified mesh. Methods Chitosan of 1 g, 1.25 g and 1.5 g were respectively dissolved into the mixture of 2% acetic acid solution, 5% glutaraldehyde solution and 50% n-heptane solution, and 2.0%, 2.5%, 3.0% chitosan mould solutions were obtained. Polypropylene meshes were modified by different concentrations of chitosan mould solutions, and then the chitosan-modified polypropylene meshes were obtained. Rabbit ADSCs were cultured with chitosan-modified polypropylene meshes for 24 h, and the adhesion behavior of cells on materials was observed. Results Chitosan-modified polypropylene meshes were harder than non-modified polypropylene meshes, and the plasticity of polypropylene meshes decreased with the concentration increase of chitosan solutions. Polypropylene mesh modified by 3.0% chitosan solution had the phenomenon of uneven coating. After co-culture for 24 h, rabbit ADSCs grew well on modified polypropylene meshes, while there was no rabbit ADSCs growing on the non-modified polypropylene meshes. Conclusion The ideal concentration of the chitosan solution for modified polypropylene mesh is 2.5%, and chitosan-modified polypropylene mesh is suitable for rabbit ADSCs adhesion.

Key words: chitosan, polypropylene mesh, rabbit adipose-derived stromal cell, adhesion