Abstract:

Objective To construct the short hairpin RNA (shRNA) of extracellular matrix metalloproteinase inducer (EMMPRIN), and investigate its influence on invasion and migration of human gastric cancer SGC-7901 cells. Methods Four groups of human EMMPRIN siRNA were designed and synthesized, double-stranded shRNA was annealed, which was then inserted into pGenesil-1 plasmid. The interference plasmid of EMMPRIN with significant silencing effect was screened by sequencing, RT-PCR and Western blotting, and was transfected into SGC-7901 cells by Lipofectamine 2000. Transwell assay was employed to determine the effect of recombinant interference plasmid on invasion and migration of SGC-7901 cells. Results The interference plasmid pshRNA-EMMPRIN was constructed and screened. After transfection, the expression of EMMPRIN mRNA and protein in SGC-7901 cells significantly decreased. Transwell assay revealed that the number of cells with invasion and migration significantly decreased in SGC-7901 cells after transfection with recombinant interference plasmid. Conclusion The downregulation of EMMPRIN expression can significantly decrease the invasion and migration of tumor cells, which lays a foundation for the further study of gene therapy of tumor with EMMPRIN.

Key words: extracellular matrix metalloproteinase inducer, short hairpin RNA, SGC-7901, invasion, migration