Table of Content

28 January 2013, Volume 33 Issue 1 Previous Issue    Next Issue
---
Original article (Basic research)
Original article (Clinical research)
Original article (Preventive medicine)
Original article (Public health administration)
Review
Brief original article
Technique and method


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Original article (Basic research)

Select

Construction and characteristics of novel PCL-PEG-chitosan nano non-viral gene delivery system WANG Bin, JIN Cai-yi, TONG Hai-jun, et al 2013, 33 (1):  1.  doi: 10.3969/j.issn.1674-8115.2013.01.001 Abstract ( 899 )   PDF (643KB) ( 1336 )   Objective To optimize the complexation condition of the novel nano non-viral gene vectors of polycaprolactone (PCL)-polyethylene glycol (PEG)-chitosan with plasmid in vitro, and evaluate the transfection activity of the complexes. Methods The minimum N/P ratio of the complexation of non-viral gene vector with plasmid carrying green fluorescent protein gene (pEGFP) was measured by gel electrophoresis in vitro. The particle sizes of the complexes under different N/P ratios and different buffering conditions were determined by dynamic light scattering method. The 293T cells were transfected with complexes of pEGFP with PCL-PEG-chitosan, and the transfection efficiency of complexes was observed under fluorescence microscope. Results The results of gel electrophoresis demonstrated that the minimum N/P ratio of complexation of PCL-PEG-chitosan with pEGFP was 4∶1, the minimum N/P ratio of complexation of PCL-chitosan with pEGFP was also 4∶1, and the minimum N/P ratio of complexation of chitosan with pEGFP was 1∶1. Dynamic light scattering test revealed that the particle sizes of the complexes of PCL-PEG-chitosan with pEGFP decreased with the increase of N/P ratios, and the particle sizes in acetate buffer system and DMEM buffer system were similar and maintained stable. Fluorescence microscopic observation indicated that the transfection efficiency of PCL-PEG-chitosan improved to some extent. Conclusion PCL modification can attenuate the complexation ability of chitosan with pEGFP, and increase the minimum complexation N/P ratio. The transfection efficiency of PCL-PEG-chitosan improves after PCL and PEG modification, and needs to be further improved. Related Articles | Metrics

Select

Aldosterone induced endothelial cell apoptosis via modulation of ACE2-Ang (1-7)-Mas receptor axis ZHANG Xia, PAN Yu, JIN Hui-min 2013, 33 (1):  6.  doi: 10.3969/j.issn.1674-8115.2013.01.002 Abstract ( 982 )   PDF (606KB) ( 1315 )   Objective To investigate the effect of aldosterone on ACE2-Ang (1-7)-Mas receptor axis of endothelial cells, and explore its association with apoptosis. Methods Human umbilical vein endothelial cells (HUVEC) cultured in vitro were divided into control group (DMEM/F12 culture medium), aldosterone group (treatment with 10, 100, 1 000 nmol/L aldosterone) and aldosterone antagonist group (100 nmol/L aldosterone+1 μmol/L aldosterone antagonist). The expression of ACE2 protein in cells was observed with immunofluorescence cytochemical staining, the expression of ACE2 receptor and Mas receptor was determined by Western blotting, the contents of AngII and Ang (1-7) protein and caspase-3 activity in the supernatant of culture fluid were measured by ELISA, and cell apoptosis was detected by flow cytometry with FITC-Annexin V/PI fluorescein staining. Results Compared with control group, the expression of ACE2 receptor and Mas receptor in cells in aldosterone group significantly decreased in a dose-dependent manner (P<0.01). In 100 nmol/L aldosterone group, the expression of ACE2 receptor and Mas receptor in cells significantly decreased in a time-dependent manner (P<0.01), while the expression of ACE2 receptor and Mas receptor in cells in aldosterone antagonist group was significantly higher than that in 100 nmol/L aldosterone group (P<0.01). The content of AngⅡand caspase-3 activity in the supernatant of culture fluid in aldosterone group significantly increased with the time, while the content of Ang (1-7) significantly decreased. The apoptosis rate in aldosterone group was significantly higher than that in control group, while the apoptosis rate in aldosterone antagonist group was significantly lower than that in aldosterone group (P<0.05). Conclusion Aldosterone may regulate ACE2-Ang (1-7)-Mas receptor axis, which may play a role in inducing endothelial cell apoptosis. Related Articles | Metrics

Select

Influence of short hairpin RNA of EMMPRIN on invasion and migration of SGC-7901 cells KANG Yu-jun, WANG Chuan, JIANG Zheng, et al 2013, 33 (1):  12.  doi: 10.3969/j.issn.1674-8115.2013.01.003 Abstract ( 902 )   PDF (1232KB) ( 1122 )   Objective To construct the short hairpin RNA (shRNA) of extracellular matrix metalloproteinase inducer (EMMPRIN), and investigate its influence on invasion and migration of human gastric cancer SGC-7901 cells. Methods Four groups of human EMMPRIN siRNA were designed and synthesized, double-stranded shRNA was annealed, which was then inserted into pGenesil-1 plasmid. The interference plasmid of EMMPRIN with significant silencing effect was screened by sequencing, RT-PCR and Western blotting, and was transfected into SGC-7901 cells by Lipofectamine 2000. Transwell assay was employed to determine the effect of recombinant interference plasmid on invasion and migration of SGC-7901 cells. Results The interference plasmid pshRNA-EMMPRIN was constructed and screened. After transfection, the expression of EMMPRIN mRNA and protein in SGC-7901 cells significantly decreased. Transwell assay revealed that the number of cells with invasion and migration significantly decreased in SGC-7901 cells after transfection with recombinant interference plasmid. Conclusion The downregulation of EMMPRIN expression can significantly decrease the invasion and migration of tumor cells, which lays a foundation for the further study of gene therapy of tumor with EMMPRIN. Related Articles | Metrics

Select

Effect of rosuvastatin on impaired angiogenesis induced by hyperglycemia WANG Fa-bin, LI Jing-bo, ZHU Wei, et al 2013, 33 (1):  19.  doi: 10.3969/j.issn.1674-8115.2013.01.004 Abstract ( 983 )   PDF (802KB) ( 1443 )   Objective To investigate the effect of rosuvastatin on impaired angiogenesis induced by hyperglycemia. Methods Primary microvascular endothelial cells of myocardium were cultured. Cells of passage one were randomly divided into low-glucose group (5.6 mmol/L glucose), high-glucose group (33.3 mmol/L glucose), rosuvastatin group (33.3 mmol/L glucose+1 μmol/L rosuvastatin) and LY294002 (phosphatidylinositol 3-kinase inhibitor) group (33.3 mmol/L glucose +1 μmol/L rosuvastatin+10 μmol/L LY294002). Matrigel, Transwell and MTT assays were used to evaluate the ability of tube formation, migration and proliferation of endothelial cells respectively. Moreover, Western blotting was employed to detect the protein expression of protein kinase B (Akt), phosphoAkt (Ser473)(p-Akt), endothelial nitric oxide synthase (eNOS) and phospho-eNOS(Ser1117)(p-eNOS). Results The ability of tube formation, migration and proliferation of endothelial cells in highglucose group was significantly lower than that in low-glucose group (P<0.05). The ability of tube formation, migration and proliferation of endothelial cells in rosuvastatin group was significantly higher than that in high-glucose group and LY294002 group (P<0.05), but was significantly lower than that in low-glucose group (P<0.05). The relative expression of p-Akt and p-eNOS protein in rosuvastatin group was significantly higher than that in high-glucose group and LY294002 group (P<0.05), but was not significantly different from that in low-glucose group (P>0.05). Conclusion Rosuvastatin can significantly improve hyperglycemia-induced impaired angiogenesis in endothelial cells, the mechanism of which may be associated with the activation of PI3K/ Akt/eNOS pathway. Related Articles | Metrics

Select

Effects of autophagy on growth inhibition of Gefitinib in non-small cell lung cancer cell lines XU Zhi-hong, GAO Bei-li, HU Jia-an 2013, 33 (1):  25.  doi: 10.3969/j.issn.1674-8115.2013.01.005 Abstract ( 1434 )   PDF (631KB) ( 1374 )   Objective To investigate the effects of autophagy on growth inhibition of Gefitinib in non-small cell lung cancer (NSCLC) cell lines. Methods The content of autophagy marker LC3II was detected by Western blotting and LC3 antibody, and whether Gefitinib could induce autophagy in NSCLC Calu6 and H460 cells were determined. H460 and Calu6 cells were transfected with enhanced green fluorescence protein (EGFP)-LC3b plasmid, and were treated with 40 μmol/L Gefitinib for 48 h respectively. The distribution of green fluorescence protein (GFP) was observed. In addition, H460 cells were treated with Everolimus, Gefitinib, and Everolimus combined with Gefitinib respectively. Ten days later, cells were stained with crystal violet, cell clonies were counted under microscope, and growth inhibition rates were calculated. Results Western blotting revealed that LC3Ⅱ was significantly accumulated after treatment with 40 μmol/L Gefitinib for 48 h, and green fluorescence was dispersedly distributed. There were significant differences in numbers of clony formation among H460 cells treated with Everolimus, Gefitinib, and Everolimus combined with Gefitinib (P<0.05), and the growth inhibition rate of Everolimus combined with Gefitinib was the highest. Conclusion Gefitinib induces autophagy in NSCLC cells, and autophagy induction enhances growth inhibition of Gefitinib on NSCLC cells. Related Articles | Metrics

Select

Characteristics of number and phenotype of mast cell precursors in hepatoma-bearing mice SONG Chuan-wang, LIAO Sheng-jun, ZHANG Gui-mei, et al 2013, 33 (1):  30.  doi: 10.3969/j.issn.1674-8115.2013.01.006 Abstract ( 1388 )   PDF (419KB) ( 1231 )   Objective To investigate the changing characteristics of number and phenotype of mast cell precursors in hepatoma-bearing mice. Methods H22 hepatoma-bearing mouse model was established. Single cells were prepared from bone marrow, spleen and peripheral normal and tumor tissues. Flow cytometry was employed to analyze the proportions of granulocyte/monocyte precursors (GMP) in bone marrow cells, basophil-mast cell progenitors (BMCP) in spleen cells, and mast cell precursors in peripheral normal and tumor tissues. In addition, the expression of Toll-like receptor-2 (TLR2) in BMCP and MCP was determined by flow cytometry. Results The proportions of GMP in bone marrow cells, BMCP in spleen cells and mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice were significantly higher than those in normal mice (P＜0.05). There was no significant difference in proportions of TLR2-positive cells in BMCP in spleen between hepatoma-bearing mice and normal mice (P＞0.05), but the proportion of TLR2-positive cells in mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice was significantly higher than that in normal mice (P＜0.05). Conclusion Mobilization of mast cells is significantly increased and there are higher proportions of mast cell precursors in bone marrow, spleen and peripheral tumor tissues in H22 hepatoma-bearing mice. But there is no significant change in expression of TLR2 in BMCP, and the change mainly takes place in mast cell precursor in peripheral tumor tissues in hepatoma-bearing mice. Related Articles | Metrics

Select

Microanatomic study on supraorbital extradural approach to cavernous sinus under endoscope YIN You-hui, WANG Hai-ping, YIN Shang-jiong, et al 2013, 33 (1):  35.  doi: 10.3969/j.issn.1674-8115.2013.01.007 Abstract ( 1218 )   PDF (840KB) ( 1466 )   Objective To investigate the microanatomic and surgical exposure of supraorbital extradural approach to cavernous sinus under endoscope, so as to provide the microanatomic basis for clinical neurosurgery. Methods Twenty fresh cadaver heads were studied using 4 mm 0° and 30° endoscopes to develop a surgical approach and identify surgical landmarks. Results The endoscopic supraobital extradural approach could be divided into 3 stages: entry into the extradural anterior cranial fossa, exposure of the middle cranial fossa and periorbita, and exposure of the superior and lateral cavernous sinus. This approach could expose superb views of the cavernous sinus microanatomy especially through the Dolence triangle, Parkinson triangle and Mullan triangle. Conclusion Endoscopic supraobital extradural approach offers excellent microanatomy of the cavernous sinus with minimal invasiveness, which is worthy of introduction. Related Articles | Metrics

Original article (Clinical research)

Select

Effect of different ovarian response after controlled ovarian hyperstimulation on pregnancy outcome of women undergoing in vitro fertilization GAO Min-zhi, SUN Zhao-gui, ZHAO Xiao-ming, et al 2013, 33 (1):  39.  doi: 10.3969/j.issn.1674-8115.2013.01.008 Abstract ( 1213 )   PDF (475KB) ( 1272 )   Objective To investigate the effect of different ovarian response after controlled ovarian hyperstimulation (COH) on pregnancy outcome of women undergoing in vitro fertilization. Methods The clinical data of 1 310 women undergoing first cycle of in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) due to male and/or tubal factor were analysed. According to the percentiles of serum E2 levels on the day of human chorionic gonadotrophin (hCG) administration, poor ovarian response group (group A, 0% to 10%), low-mid ovarian response group (group B, 11% to 25%), mid ovarian response group (group C, 26% to 75%), mid-high ovarian response group (group D, 76% to 90%) and high ovarian response group (group E, 91% to 100%) were divided. The numbers of oocytes retrieved, embryo development and pregnancy outcomes were compared among groups. Results The rate of clinical pregnancy was 44.73% for all the patients, and the implantation rate and abortion rate were 30.05% and 3.73% respectively. The numbers of MⅡ oocytes significantly increased from group A to group E (P<0.01). The numbers of goodquality embryos increased from group A to group E, and there were significant differences among groups (P<0.01) except for group D and group E. The numbers of embryos transfered in group A, group D and group E were significantly smaller than those in group B and group C (P<0.01). The cycle cancellation rate was the lowest in group C, and the cycle cancellation rates in group D and group E were significantly higher than those in group B and group C (P<0.05 or P<0.01). The pregnancy rates and implantation rates increased form group A to group C, and those decreased from group C to group E. The pregnancy rate and implantation rate in group C were significantly higher than those in group A and group E (P<0.05 or P<0.01). There was no significant difference in the abortion rate among groups (P>0.05). Conclusion Both low ovarian response and high ovarian response are detrimental to pregnancy outcome of women undergoing in vitro fertilization. Related Articles | Metrics

Select

Variation of drug susceptibility of Acinetobacter baumannii within 6 years and countermeasure analysis XIAO Shu-zhen, MI Chen-rong, HAN Li-zhong, et al 2013, 33 (1):  46.  doi: 10.3969/j.issn.1674-8115.2013.01.009 Abstract ( 1157 )   PDF (668KB) ( 1259 )   Objective To investigate the clinical distribution and variation of drug resistance of Acinetobacter baumannii, and provide reference for antibiotic drug usage and infection control. Methods A total of 2 397 clinical isolates of Acinetobacter baumannii with result of drug sensitivity test were collected from Department of Clinical Microbiology of the hospital, and the distribution of isolates of Acinetobacter baumannii in clinical departments was analyzed. The sensitivity of Acinetobacter baumannii to 13 commonly used antibiotic drugs was determined by K-B method. Results There was no significant change in the distribution of clinical isolates of Acinetobacter baumannii within 6 years. The most common locations of Acinetobacter baumannii were Department of Burn, Department of Cardiothoracic Surgery, Department of Surgery, Department of Respirology, Department of Hematology and Department of Geriatrics. The sensitivity of Acinetobacter baumannii to commonly used antibiotic drugs generally descended. Isolates from section for outpatients and internal medicine departments were more sensitive to third-generation cephalosporin, fluoroquinolone and aminoglycosides, while strains from emergency department, surgical departments and intensive care unit (ICU) had a higher rate of drug resistance. Conclusion The sensitivity of Acinetobacter baumannii to antibiotic drugs decreases year by year, which should be paid more attention. Doctors should choose proper antibiotic drugs for different departments based on antimicrobial susceptibility results. Infection monitoring should be enhanced in hospitals to reduce the spread of drug-resistant bacteria. Related Articles | Metrics

Select

Relationship among symptoms, endoscopic classification and pathological characteristics of Barrett esophagus YIN Xia, XU Yan-li, ZHOU Jun, et al 2013, 33 (1):  50.  doi: 10.3969/j.issn.1674-8115.2013.01.010 Abstract ( 1522 )   PDF (882KB) ( 1391 )   Objective To investigate the relationship among symptoms, endoscopic classification and pathological characteristics of Barrett esophagus (BE). Methods A total of 7 150 patients undergoing gastroscopic examinations for upper gastrointestinal tract symptoms were investigated with gastroesophageal reflux disease (GERD) questionnaire (GerdQ), and GERD was diagnosed when GerdQ sum score was ≥8. Among those diagnosed as BE by gastroscopic examinations, long-segment BE (LSBE) group (n=27) and short-segment BE (SSBE) group (n=65) were divided according to the morphology under gastroscope, and 78 patients with cardia intestinal metaplasia (CIM) were served as controls (CIM group). The esophagitis was observed under endoscope in each group. The biopsy specimens were obtained, and the histopathological features, dysplasia and helicobacter pylori (H.pylori) infection were observed with HE, AB and Giemsa staining respectively. Results Endoscopic examinations revealed that the prevalence of BE was 1.29%. The prevalences of GERD in LSBE group, SSBE group and CIM group were 92.59%, 69.23% and 41.03% respectively, the prevalences of esophagitis were 100.00%, 78.46% and 29.49% respectively, and the prevalences of dysplasia were 66.67%, 24.62% and 10.26% respectively, with significant differences among groups (P＜0.05 or P＜0.01). The prevalence of intestinal metaplasia in LSBE group was 74.07%, which was significantly higher than that in SSBE group (50.77%)(P＜0.05). The Hp infection rates in LSBE group and SSBE group were 18.52% and 21.54% respectively, which were significantly lower than that in CIM group (47.44%)(P＜0.05). Crypt disarray and atrophy, squamous epithelium overlying columnar crypts, intestinal metaplasia, esophageal ducts and multilayered epithelium were more likely to be found in patients with BE group than in CIM group (P＜0.05), and squamous epithelium overlying columnar crypts and esophageal ducts were exclusively found in patients with BE. Conclusion GERD, esophagitis, specialized intestinal metaplasia and dysplasia are more likely to occur in patients with BE, especially in those with LSBE. The Hp infection rate in patients with BE is low. Crypt disarray and atrophy, squamous epithelium overlying columnar crypts, intestinal metaplasia, esophageal ducts and multilayered epithelium are associated with the diagnosis of BE. Squamous epithelium overlying columnar crypts and esophageal ducts are exclusively in patients with BE. Related Articles | Metrics

Select

Study of hepcidin level in bone marrow in patients with myelodysplastic syndromes GU Shu-cheng, SONG Xiao-li, ZHAO You-shan, et al 2013, 33 (1):  56.  doi: 10.3969/j.issn.1674-8115.2013.01.011 Abstract ( 1102 )   PDF (481KB) ( 1174 )   Objective To determine the role of hepcidin and related factors in evaluation of iron overload in patients with myelodysplastic syndromes (MDS). Methods Sixty-four patients with MDS were enrolled. The hepcidin levels in peripheral blood and bone marrow were measured by ELISA, the expression of growth differentiation factor 15 (GDF15) and twisted gastrulation 1 (TWSG1) mRNA was detected by Real-Time PCR, T lymphocyte subtypes (CD4+ and CD19+ lymphocytes) and T lymphocyte polarization were determined by flow cytometry, the deposition of iron in heart and liver was examined through magnetic resonance imaging T2* (MRI T2*), and serum ferritin (SF), C-reactive protein (CRP) and erythropoietin (EPO) levels were measured. Results There was no significant difference between hepcidin level in peripheral blood and that in bone marrow (P=0.134). Stratified according to WHO 2008 subtypes, the hepcidin level in patients with refractory anemia with ringed sideroblasts (RARS) was the lowest [（105.40±5.13）ng/mL], and that in patients with refractory anemia with excess blasts (RAEB) was the highest [（335.71±25.16）ng/mL], and there were significant differences among groups (P=0.041). Stratified according to IPSS and WPSS, there were significant differences in hepcidin levels between low-risk group and high-risk group in two systems respectively (P<0.05 and P<0.01). The SF level and expression of GDF15 mRNA in WPSS highrisk group were significantly higher than those in WPSS low-risk group (P<0.05), while there was no significant difference between IPSS low-risk group and IPSS high-risk group (P>0.05). Stratified according to T lymphocyte subtype and polarization, the hepcidin level in CD4+ high-expression group was higher than that in normal expression group (P<0.05), but there was no significant difference in the hepcidin levels between CD19+ high-expression group and normal expression group (P=0.206), and the hepcidin level in Th1/Th2>70.6 group was higher than that in Th1/Th2≤70.6 group (P<0.001). Stepwise regression analysis revealed that liver iron concentration (LIC, measured by MRI T2*), instead of SF and cardiac T2*, was correlated with hepcidin (r=0.582, P<0.001). Conclusion Inflammation has a significant impact on hepcidin expression, and the activation and polarization of T lymphocytes may partially participate in the mechanism. T2*MRI outperformed SP in evaluation of iron overload in patients with MDS. Related Articles | Metrics

Select

Preliminary surgical experience of minimally invasive device closure of perimembranous ventricular septal defects WANG Shun-min, XU Zhi-wei, LIU Jin-fen, et al 2013, 33 (1):  62.  doi: 10.3969/j.issn.1674-8115.2013.01.012 Abstract ( 1207 )   PDF (809KB) ( 1187 )   Objective To summarize the clinical experience of minimally invasive perventricular device closure of perimembranous ventricular septal defects (VSD), and explore the surgical techniques and indications. Methods Forty-eight patients with perimembranous VSD underwent perventricular device closure with inferior sternotomy. Restrictive perimembranous VSD were found in all the patients. The basal diameters of VSD ranged between 2 mm and 9 mm, and the diameters of the occlusion device waist ranged between 4 mm and 10 mm. Concentric occluders were used in 45 patients, and eccentric occluders were used in 3 patients. The occlusion device was deployed through right ventricle under echocardiographic guidance. Results Forty-five patients were stable in heart function after operation. Patients were followed up for 1 to 6 months, and there was no position shift of the occlusion devices, arrhythmia or residual shunt. Arrhythmia occurred in the other 3 patients after operation, including ventricular premature beat in 1 patient and atrioventricular block in the other 2 patients. All of these three patients recovered after treatment. Conclusion The perventricular device closure of perimembranous VSD with inferior sternotomy appears to be a favorable choice for the treatment of VSD, while the surgical indications should be carefully managed. Related Articles | Metrics

Select

Correlation of neuropathic pain with depression disorder and anxiety disorder NI Xue-jun, DU Dong-ping 2013, 33 (1):  67.  doi: 10.3969/j.issn.1674-8115.2013.01.013 Abstract ( 1621 )   PDF (285KB) ( 1346 )   Objective To investigate the correlation of neuropathic pain with depression disorder and anxiety disorder. Methods Thirty-six patients who were initially diagnosed as neuropathic pain were included (case group), and were treated with amitriptyline, opioids and nerve block in necessities. Assessment with visual analogue scale (VAS), Zung self-rating anxiety scale (SAS) and Zung self-rating depression scale (SDS) was performed before treatment and 2 weeks and 4 weeks after treatment. Besides, 15 healthy volunteers were served as controls, and assessment with SAS and SDS was conducted. Results The duration of neuropathic pain of all patients in case group was more than 3 months. The degree of pain was positively related to the prevalences of depression disorder and anxiety disorder in case group (P<0.01), and the prevalences of depression disorder and anxiety disorder in case group were significantly higher than those in control group (P<0.01). Two weeks and 4 weeks after treatment in case group, the pain was effectively relieved (P<0.05), the degree of pain was also positively related to the prevalences of depression disorder and anxiety disorder (P<0.05), and the prevalences of depression disorder and anxiety disorder were also significantly higher than those in control group (P<0.05). Conclusion Patients with neuropathic pain are usually found to experience anxiety disorder and depression disorder. With the relief of pain, anxiety disorder and depression disorder may be relieved, but can not be completely eliminated. Related Articles | Metrics

Select

Influence of transfer medium EmbryoGlue on clinical outcome of embryo transfer ZHANG Jian-rui, GUAN Yi-chun, ZHANG Lu-wen, et al 2013, 33 (1):  71.  doi: 10.3969/j.issn.1674-8115.2013.01.014 Abstract ( 1224 )   PDF (281KB) ( 1398 )   Objective To investigate the influence of transfer medium EmbryoGlue on pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET). Methods Patients undergoing IVF-ET were enrolled, and were divided into EmbryoGlue group (EmbryoGlue as transfer medium, 232 cycles) and G-2 group (G-2 as transfer medium, 268 cycles). The clinical parameters and pregnancy outcomes were compared between groups. Results There was no significant difference in the age, duration of infertility, number of unsuccessful cycles, thickness of endometrium, number of good quality embryo, mean number of embryo transfer between groups (P>0.05). The clinical pregnancy rate, implantation rate, multiple pregnancy rate in EmbryoGlue group were significantly higher than those in G-2 group (P<0.05). The prevalence of ectopic pregnancy in EmbryoGlue group was significantly lower than that in G-2 group (P<0.05). Conclusion As an embryo transfer medium, EmbryoGlue can improve the pregnancy rate and implantation rate of IVF-ET. Related Articles | Metrics

Select

Survey on diet and vitamin C nutrition status in patients with type 2 diabetes in community ZHANG Pei-hong, YAO Bao-dong, TANG Yu-ying 2013, 33 (1):  75.  doi: 10.3969/j.issn.1674-8115.2013.01.015 Abstract ( 1115 )   PDF (251KB) ( 1327 )   Objective To investigate the diet and vitamin C nutrition status in patients with type 2 diabetes, and provide reference for the supplement of antioxidant nutrients in the treatment of diabetes. Methods Sixty-eight patients with type 2 diabetes (type 2 diabetes group) and 136 healthy people (control group) from Shanghai Ruijin Community were selected, diet nutrition was investigated, and 4-h vitamin C urinary load test was conducted. Results The results of 4-h vitamin C urinary load test suggested that 10.3% of patients in type 2 diabetes group were in insufficient status of vitamin C, which was significantly higher than that in control group (0.7%)(P<0.05). Diet survey demonstrated that daily vitamin E intake in both groups was higher than the recommended level (14 mg), but there was no significant difference between two groups (P>0.05). Besides, there was no significant difference in vitamin C intake between two groups (P>0.05). Conclusion Patients with type 2 diabetes may be in poor nutrition status of vitamin C, and supplement of antioxidant nutrients including vitamin C should be attached great importance in the treatment of diabetes. Related Articles | Metrics

Original article (Preventive medicine)

Select

Mediating effect of subjective well-being on attitude towards commit suicide and meaning in life in poverty-stricken university students XIE Xing-li, ZOU Bing 2013, 33 (1):  78.  doi: 10.3969/j.issn.1674-8115.2013.01.016 Abstract ( 1395 )   PDF (409KB) ( 1438 )   Objective To investigate the relationship between attitude towards commit suicide and meaning in life in poverty-stricken university students, and explore the mediating effect of subjective wellbeing on the development of attitude towards commit suicide. Methods Stratified random sampling was adopted to select 1 100 students from 5 universities, and the survey on attitude towards commit suicide and meaning in life was conducted. Questionnaire of suicide attitude (QSA), meaning in life questionnaire (MLQ), purpose in life test (PIL) and index of well-being (IWB) were employed, and the attitude towards commit suicide and related factors in poverty-stricken university students were analyzed. Structural equation model was constructed to explore the mediating effect of subjective well-being on attitude towards commit suicide and meaning in life. Results A total of 1 038 effective questionnaires were recovered, with the response rate of 94.4%. There were 316 poverty-stricken university students, and the other 722 were non-poverty-stricken university students. The scores on subjective well-being, attitude towards people with commit suicide and life experience in poverty-stricken university students were significantly lower than those in non-poverty-stricken university students (P<0.05). Among poverty-stricken university students, female students had significantly higher scores than male students in attitude toward families of people with commit suicide (P<0.05), while male students had significantly higher scores than female students in pursuit of meaning in life, purpose in life and sense of ownership (P<0.05). The attitude towards commit suicide was closely related to meaning in life and subjective well-being in poverty-stricken university students (P<0.05, P<0.01). Subjective well-being partially had the mediating effect on meaning in life and attitude towards commit suicide. Conclusion Meaning in life and subjective well-being may have important effect on the attitude towards commit suicide in poverty-stricken university students, and individualized life education should be conducted in universities. Related Articles | Metrics

Select

Relationship between afternoon nap and nocturnal sleep in middle and old aged community residents DAI Fei, XU Wen-qian, XU Xiao-yi, et al 2013, 33 (1):  84.  doi: 10.3969/j.issn.1674-8115.2013.01.017 Abstract ( 1164 )   PDF (329KB) ( 1409 )   Objective To investigate the relationship between afternoon nap and nocturnal sleep in middle and old aged community residents. Methods A total of 478 residents aged between 45 and 80 years were selected for face-to-face survey by cluster sampling method from 5 communities in 2 districts of Shanghai from September 2010 to March 2011. Pittsburgh sleep quality Index (PSQI) questionnaire was employed to evaluate the sleeping quality, Montreal cognitive assessment (MoCA) questionnaire was adopted to the assess the cognition function, and self-designed questionnaire was used to obtain the basic information of residents. According to afternoon nap frequencies, residents were divided into frequent afternoon nap group (≥3 times/week), occasional afternoon nap group (1 to 2 times/week) and control group (≤3 times/month). Results There were 443 valid questionnaires, with the effective rate of 92.68%. The mean age and waist circumference in frequent afternoon nap group were significantly higher than those in control group (P<0.01), but the cognition score in frequent afternoon nap group was significantly lower than that in control group (P<0.01). There were significant differences in the degree of education, employment status and self-report rate of chronic diseases between frequent afternoon nap group and control group (P<0.05 or P<0.01). After adjustment by factors such as age, degree of education, employment status, waist circumference, self-report rate of chronic diseases and cognition status, the sleep disorder score, daytime function score and total PSQI score in frequent afternoon nap group were significantly higher than those in occasional afternoon nap group and control group (P<0.05 or P<0.01), the total bed time in frequent afternoon nap group and occasional afternoon nap group was significant less than that in control group (P<0.01), and there was no significant difference in sleep latency, sleep duration and sleep efficiency among three groups (P>0.05). Conclusion The increase of afternoon nap frequency is closely associated with the poor nocturnal sleep quality of middle and old aged people, while there is no sufficient evidence that afternoon nap frequency is related to nocturnal sleep time pattern. Related Articles | Metrics

Original article (Public health administration)

Select

Analysis of research trends of nursing education in China TANG Hong-mei, CEN Jin 2013, 33 (1):  89.  doi: 10.3969/j.issn.1674-8115.2013.01.018 Abstract ( 1142 )   PDF (344KB) ( 1296 )   Objective To summarize the research trends of nursing education in China in the past years, and analyse the reform and development trends of nursing education. Methods Literatures published between January 2003 and December 2011 were retrieved with “education, nursing” as the subject terms in China Biology Medicine disc. The number of enrolled literatures, affiliations of authors, journal sources of literatures and themes of literatures were statistically analyzed. Results A total of 28 622 literatures were obtained. The number of enrolled literatures on nursing education had significantly increased since 2006, and reached 6 900 in 2011. The affiliations of authors were mainly vocational schools, medical colleges and hospitals. The journal sources included nursing related journals, clinical medicine journals and medical education journals. The themes of literatures focused on nursing education objectives, curriculum setting, teaching methods, and education reform and assessment. Conclusion The research trends of nursing education are associated with development of multi-level nursing training objectives, nursing database, localized courses, diversified teaching methods and standard evaluation system for nursing education. Related Articles | Metrics

Select

Validity testing of working well-being scale for young and middle-aged clinician CHEN Wei, FEI Jian, NIU Wen-quan, et al 2013, 33 (1):  94.  doi: 10.3969/j.issn.1674-8115.2013.01.019 Abstract ( 1032 )   PDF (358KB) ( 1192 )   Objective To evaluate the validity of working well-being scale, and provide a quantitative tool for assessment of factors related to young and middle-aged clinician. Methods A total of 250 young and middle-aged clinician aged between 30 and 45 were recruited from three comprehensive hospitals affiliated to Shanghai Jiaotong University School of Medicine by stratified cluster random sampling, and were asked to complete the working well-being scale, including mood experience section and working well-being cognition section. The construct validity of the scale was assessed by principal components analysis and factorial validity analysis. Results There were 246 effective questionnaires, with the effective rate of 98.4%. The Cronbach´s alpha coefficient and content validity index (CVI) of working well-being scale were 0.872 and 0.861 respectively. Considering that the scale was composed of mood experience section and working well-being cognition section, principal components analysis was performed in each section separately. Mood experience section was constituted by 2 main factors: positive mood and negative mood. Principal components analysis indicated that the explanation rate of total variance of original factors reached 59.94%, and the explanation rate of items reached 88.9%. Cognition section was constituted by eight main factors: pay and welfare, reward and approval, job occupation, career development, colleague, supervisor, working routine, and interpersonal coordination. The explanation rate of total variance of original factors reached 61.54%, and the explanation rate of items reached 83.3%. Conclusion The working well-being scale has acceptable construct validity, and can be used for assessment of working well-being in medical staff after modification. Related Articles | Metrics

Review

Select

Research progress of Wnt signaling pathway in regulating osteogenic differentiation of mesenchymal stem cells CHEN Xiao-jing, GAO Yan-hong 2013, 33 (1):  99.  doi: 10.3969/j.issn.1674-8115.2013.01.020 Abstract ( 1475 )   PDF (349KB) ( 1577 )   Wnt signaling pathway has been the focus of medical research as it plays a significant role in regulating the growth, development and differentiation of cells. Recent studies have revealed that Wnt signaling pathway may play an important role in regulating the osteogenic differentiation of mesenchymal stem cells (MSCs), the mechanism of which has been the hotspot of bone tissue engineering and provides a new way for the treatment of diseases such as osteoporosis. The research progress of Wnt signaling pathway in regulating osteogenic differentiation of MSCs is reviewed in this paper. Related Articles | Metrics

Select

Research progress of imaging receptor tyrosine kinase targeted drugs by radionuclide ZHANG Xie-yi, SONG Shao-li, HUANG Gang 2013, 33 (1):  104.  doi: 10.3969/j.issn.1674-8115.2013.01.021 Abstract ( 1386 )   PDF (357KB) ( 1363 )   Transmembrane receptor tyrosine kinase signalling triggers cell proliferation, apoptosis suppression and neovasculature recruitment. Transmembrane receptor tyrosine kinases are overexpressed in many malignancies, which have become the molecular targets for an increasing number of anticancer drugs. For example, monoclonal antibodies and small-molecule tyrosine kinase inhibitors have been clinically proved to boost the therapeutic effect and increase the survival of patients. However, the targeted drugs are clinically limited by specific target and high price, and the early monitoring of therapeutic effects of targeted drugs is important for the design of clinical regimen and reduction of adverse effects and medical expense. The imaging of receptor tyrosine kinase targeted drugs by radionuclide has been clinically employed in the monitoring of therapeutic effects of targeted drugs, and the research progress of this application is reviewed in this paper. Related Articles | Metrics

Select

Research progress of pluripotent stem cells and kidney regeneration HUANG Jia-hui, WANG Yang, WANG Gong-xian 2013, 33 (1):  109.  doi: 10.3969/j.issn.1674-8115.2013.01.022 Abstract ( 2059 )   PDF (377KB) ( 1452 )   Although renal transplantation has been proved to be an effective therapeutic strategy for most patients with end-stage renal failure, it is associated with some limitations, including the shortage of donor organs, immune rejection and a lifelong immunosuppressive regimen. Pluripotent stem cells, especially embryonic stem cells and induced pluripotent stem cells, can differentiate into any cell types in the body and show a good prospect of application on tissue and organ regeneration. The research progress of pluripotent stem cells and kidney regeneration is reviewed in this paper. Related Articles | Metrics

Select

Research progress on selenium binding protein 1 as a novel tumor biomarker ZHANG Chen-jing, PAN Chi, PAN Wen-sheng, et al 2013, 33 (1):  114.  doi: 10.3969/j.issn.1674-8115.2013.01.023 Abstract ( 1475 )   PDF (308KB) ( 1378 )   Selenium binding protein 1 (SBP1) has been shown to bind selenium covalently and is expressed in a variety of tissues and cell lines. Selenium is incorporated into selenoproteins that have a wide range of pleiotropic effects, including its important function in prevention of cancer progression. Recent studies have revealed that SBP1 may not only exert tumor suppressive function, but also become a novel biomarker for predicting prognosis and guiding personalized therapeutic strategies. This paper briefly reviews the studies of SBP1 as a novel biomarker and tumor suppressor in the progression of various cancers. Related Articles | Metrics

Brief original article

Select

Diagnostic significance of expression of CK7 and S100A1 in chromophobe renal cell carcinoma and renal oncocytoma XUAN Bei-lei, CHEN Yi-ming, LIU Qiang 2013, 33 (1):  118.  doi: 10.3969/j.issn.1674-8115.2013.01.024 Abstract ( 1114 )   PDF (470KB) ( 1241 )   Objective To investigate the expression and diagnostic significance of CK7 and S100A1 in chromophobe renal cell carcinoma (chRCC) and renal oncocytoma. Methods The expression of CK7 and S100A1 in 22 cases of chRCC and 20 cases of renal oncocytoma was determined by immunohistochemical staining. Results Positive expression of CK7 was observed in 20 cases (90.9%) of chRCC and 2 cases (10.0%) of renal oncocytoma (P=0.000). Positive expression of S100A1 was observed in 14 cases (63.6%) of chRCC and 19 cases (95.0%) of renal oncocytoma (P=0.003). Conclusion Immunohistochemical staining of CK7 and S100A1 may be helpful for differential diagnosis of chRCC and renal oncocytoma. Related Articles | Metrics

Technique and method

Select

Tissue precise spatial structure determination with ultra-thin cutting high-resolution imaging technology Lü Chun-Mei, MENG Jun, LI Xiao-lin, et al 2013, 33 (1):  121.  doi: 10.3969/j.issn.1674-8115.2013.01.025 Abstract ( 979 )   PDF (1126KB) ( 1321 )   Objective To establish a high-resolution three-dimensional imaging system with the Z-axis resolution improvement by ultra-thin cutting technology for tissue precise structure analysis. Methods Tissue samples were embedded with LRWhite resin and polymerized, which were cut into continuous slices of 50 nm-1 000 nm with diamond knife. Thereby the Z-axis resolution of 50 nm-1 000 nm was obtained. The semi-thin cutting or ultra-thin cutting slices were stained with methylation blue and basic fuchsin or labeled with antibody for structure illustration by automatic microscope. The high-resolution three-dimensional tissue spatial information was obtained with two-dimensional stitching and three-dimensional reconstruction. Results Using the established highresolution microscopic imaging technology, the fine structure of human normal colon tissues and mouse liver were observed. In combination with staining and antibody labeling technology, the distribution of tubulin in mouse brain tissues was observed by high-resolution three-dimensional imaging. Conclusion The established high-resolution three-dimensional imaging technology is applicable to the precise spatial structure of all kinds of tissues, and provides a solution to the Z-axis resolution for ultra-high-resolution imaging technology. Related Articles | Metrics

Select

Study on favorable setup of flow cytometry sorter FACSAriaⅡ in sorting GFP positive population of breast cancer cell line MCF-7 GUO Chen-zhi, SHI Gui-ying 2013, 33 (1):  127.  doi: 10.3969/j.issn.1674-8115.2013.01.026 Abstract ( 1689 )   PDF (512KB) ( 1860 )   Objective To sort green fluorescent protein (GFP) positive population of breast cancer cell line MCF-7 by flow cytometry sorter FACSAriaⅡ, and investigate the effect of different setup on the purity, yield and viability of collected cells. Methods GFP positive population of MCF-7 cells was sorted by 70 μm, 85 μm and 100 μm nozzles and 4-way purity, purity and yield sort modes respectively. The yield was calculated with cell counting, the purity was analyzed by flow cytometry, and the viability was determined by trypan blue staining. Results FACSAriaⅡ gained purity of more than 99% with all three nozzles. The yields of 70 μm, 85 μm and 100 μm nozzles were (37.67±2.05)%, (56.33±2.62)% and (43.33±0.47)% respectively, while the percentages of live cells were (88.23±0.97)%, (91.87±1.05)% and (95.33±0.90)% respectively. Sorting cells by 4-way purity, purity and yield sort modes with 85 μm nozzle, the yields were (51.00±2.45)%, (67.33±2.05)% and (74.00±0.82)% respectively, and the purities were 99.6%, 99.3% and 96.2% respectively. Conclusion In sorting GFP positive population of breast cancer cell line MCF-7 by FACSAriaⅡ, 85 μm nozzle and purity sort mode may be the favorable setup. Related Articles | Metrics