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Monocyte chemoattractant protein 1 helping wound healing in diabetic mice

PENG Yin-bo1, LU Hua-xiang1, YAO Min1, FANG Yong1, GU Chuan1,2   

  1. 1.Department of Burn and Plastic Surgery, Institute of Trauma, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China; 2.Department Plastic and Reconstructive Surgery, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2013-12-28 Published:2014-01-02
  • Supported by:

    Foundation for Young Scientists of Shanghai Municipal Health Bureau, 2008Y063;  Foundation of Shanghai Jiaotong University School of Medicine, 11XJ21051; Young Scientists Project of National Natural Science Foundation, 81101431

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Abstract:

Objective To assess effects of exogenous monocyte chemoattractant protein 1 (MCP-1) in wound healing of diabetic mice. Methods Sixty BSK type 2 diabetic mice were randomly divided into MCP-1 treated group and control group (30 in each). An excision wound down to the deep fascia, measuring 1 cm×1 cm, was made on the back of mice.  The MCP-1 group mice were injected with MCP-1 (50 ng/d) for 3 d, and the control group mice received equal volume of PBS. The rate of wound healing, deposition of hydroxyproline (OHP), new blood vessels, and the level of vascular endothelial growth factor (VEGF) were assessed on day 3, 5, 7, 10, and 14 after injury. Results Since day 5, compared to control group, the rate of wound healing and deposition of OHP were higher in MCP-1-treated group (P<0.05). On day 5 and 7,  the new blood vessels per high power field were 5.25±0.83 vs 3.2±0.52 and 7.46±0.69 vs 4.8±0.81 respectively (P<0.01) in MCP-1-treated group and control group. Additionally, the levels of VEGF in MCP-1 group were significantly higher than those in control group on day 5, 7, and 10. Conclusion Exogenous MCP-1 may promote diabetic wound healing by enhancing the production of collagen and angiogenesis.

Key words: wound healing, monocyte chemoattractant protein 1, angiogenesis, collagen synthesis, diabetes