• Original article (Basic research) • Previous Articles     Next Articles

Effects of Bag1 expression inhihition on cell growth and chemotherapy sensitivity in gastric cancer

MA Fei1,3, ZHENG Lei-zhen1, GU Jian-chun1, LI Xiao-ping1, ZHANG Ming-di2, WANG Ya-jie3   

  1. 1.Department of Oncology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3.Department of Oncology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    Science and Technology Foundation of Shanghai Jiao Tong University School of Medicine, 12XJ22004

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Abstract:

Objective To investigate the effects of Bag1 expression level on the growth of gastric cancer cells and chemo-sensitivity to 5-fluorouracil (5-FU). Methods The expression levels of Bag1 in five different gastric cancer cell lines were determined by Real-Time PCR and Western blotting. Cells with highly expressed Bag1 were selected and the basic expression level of Bag1 was down-regulated by the lentiviral shRNA. The efficiency of Bag1 silencing was confirmed by Real-Time PCR and Western blotting. The effects of the Bag1 knockdown on cell proliferation and chemo-sensitivity to 5-FU were detected by CCK-8 method. Results The expression levels of mRNA and protein of Bag1 were significantly increased in SGC7901 and KATO-Ⅲ cells. Efficient knockdown of Bag1 resulted in suppressing the proliferation of SGC7901 and KATO-Ⅲ cells and significantly enhancing the chemo-sensitivity to 5-FU. Conclusion Knockdown of Bag1 can significantly suppress the proliferation of gastric tumor cells and enhance chemo-sensitivity to 5-FU. Bag1 could be a new therapeutic target for chemotherapy of GC.

Key words: gastric cancer, Bag1, cell growth, chmotherapy, sensitivity