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Preliminary study on correlation between eukaryotic initiation factor 5A and spontaneous abortion

QIN Xiao-li, LIU Xiao-rui, TIAN Fu-ju, LIN Yi   

  1. Department of Gynecology and Obstetrics, International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2015-04-28 Published:2015-04-29
  • Supported by:

    National Natural Science Foundation of China， 31171439； National Funds for Distinguished Young Scientists of China， 81125004

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Abstract:

Objective To investigate the correlation between eukaryotic initiation factor 5A (eIF5A) and spontaneous abortion. Methods Expressions of eIF5A-1 and eIF5A-2 in mouse uterine tissue of spontaneous abortion model and normal pregnancy controls and in decidua tissue of patients undergoing spontaneous abortion and artificial abortion were detected by immunofluorescence staining, real-time PCR, and Western blotting analysis. Results Both eIF5A-1 and eIF5A-2 were detected in mouse uterine tissue and human decidua tissue, but the expression of eIF5A-1 was significantly higher than that of eIF5A-2. The mRNA and protein expressions of eIF5A-1 and eIF5A-2 of the spontaneous abortion group were lower than those of the control group. Conclusion The down-regulation of eIF5A expression may be relevant to the occurrence and development of spontaneous abortion.

Key words: eukaryotic initiation factor 5A, spontaneous abortion, animal model