Journal of Shanghai Jiao Tong University (Medical Science) ›› 2022, Vol. 42 ›› Issue (5): 583-590.doi: 10.3969/j.issn.1674-8115.2022.05.005

• Basic research • Previous Articles     Next Articles

Establishment and evaluation of standardized steps for building a guinea pig model of auditory brainstem implantation

ZHOU Xiang1,2(), PAN Jinxi1,2, ZHANG Qinjie1,2, LI Yun1,2,3, CHEN Ying1,2,3, TAN Haoyue1,2, PENG Fei2, HUANG Sui4, TAN Zhiping4, WU Hao1,2, JIA Huan1,2()   

  1. 1.Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
    2.Ear Institute, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai 200125, China
    3.Hearing Implantation Center, Shanghai Ninth People's Hospital, Shanghai 200125, China
    4.Nurotron Biotechnology Co. , Ltd. , Hangzhou 311100, China
  • Received:2022-03-27 Accepted:2022-04-15 Online:2022-05-28 Published:2022-05-28
  • Contact: JIA Huan;
  • Supported by:
    National Natural Science Foundation of China(82000990);Shanghai Huangpu District Industrial Support Fund(XK2019015);Shanghai Talent Development Fund(2019047);Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases(14DZ2260300);Collaborative Innovation Project for Translational Medicine at Shanghai Jiao Tong University School of Medicine(TM202011)

Abstract: Objective

·To establish the guinea pig model of auditory brainstem implant (ABI) by modified transbulla-labyrinthine approach and assess its effectiveness and reliability.


·Twenty healthy adult male albino guinea pigs with normal hearing underwent ABI surgery via modified transbulla-labyrinthine approach. During the operation, the dorsal cochlear nucleus (DCN) of the brainstem was exposed laterally and implanted with a special ABI electrode array for guinea pigs. The evoked auditory brainstem response (eABR) was recorded from DCN and compared with the eABR waveform elicited from cochlear intraoperativly. After the electrode was implanted, the eABR waveform at DCN was recorded before euthanasia at 48 h, 7 d, 30 d and 180 d after operation. Then the brain tissue containing the electrode was harvested and the electrode neural-interface was evaluated by hematoxylin-eosin staining, immunofluorescence staining and q-PCR.


·The eABR was successfully recorded at DCN from all animals during surgery, and the death occurred at 36 h to 48 h after operation. The waveform morphology of eABR at DCN was similar to that at cochlea. The latency of wave Ⅲ in eABR at DCN [ (0.84 ± 0.22)ms] was shorter than that of wave Ⅳ in EABR at cochlea (n=19), and the threshold difference between them was (0.11±0.14) mA (n=19). Compared with the intraoperative eABR at DCN, the average threshold increased by (0.10±0.10) mA (n=16), and the average delay of wave Ⅲ latency was (0.15±0.21) ms (n=16). Histological morphology analysis showed that the contact surface of cochlear nucleus around the electrode showed vacuolar changes at 48 h post-operation, and increased glial components were observed at the edge of DCN at 48 h, 7 d and 180 d post-operation. Results of q-PCR showed IL-1β and MMP9 were upregulated at 48 h and 7 d post-operation compared with the unimplanted electrode group, but there was no significant difference.


·The modeling success rate of established ABI-implanted guinea pig model is high and have outstanding repeativeness, and the local gliosis is preliminarily observed after implantation.

Key words: auditory brainstem implantation (ABI), animal model, guinea pig, auditory electrophysiology, histological morphology

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