Abstract:

Objective To investigate the effects of lenalidomide on the biological and immunosuppressive functions of human bone marrow mesenchymal stem cells (MSCs). Methods MSCs were cultured by lenalidomide of 10 μmol/L and variations of proliferation, phenotype, and inhibition of T-cell proliferation of MSCs before and after being treated by lenalidomide were analyzed. The proliferation rate was detected by the CCK-8 assay. The proliferation cycle, phenotype, and inhibition of CD3+Tcell proliferation of MSCs were detected by the flow cytometry. Results The growth rate, proliferation, and immunosuppressive functions of MSCs were not affected by the intervention of lenalidomide. Compared with the immunophenotyping of MSCs before being treated by lenalidomide, that of MSCs after being treated by lenalidomide was different. The mean fluorescence intensity (MFI) of CD29 and CD73 increased and the MFI of CD44 decreased. Conclusion Lenalidomide can influence the expression of surface molecules of MSCs in vitro, which may contribute to the antitumor effect without affecting the growth, proliferation, and immunosuppressive functions of MSCs.

Key words: mesenchymal stem cells, lenalidomide, immunophenotyping, immunosuppressive