Abstract:

Objective   To explore the effect of bone marrow mononuclear cells (BMMNCs) transplantation on the treatment of mice with myocardial infarction (MI) by Millar pressure-volume conductance catheter system and echocardiography. Methods  A total of 30 mice were randomly divided into the MI group (n=10), MI+BMMNCs treatment group (BMMNCs group, n=10), and sham operation group (n=10). MI model was established for mice of MI and BMMNCs groups. Mice of the BMMNCs group were injected with BMMNCs through caudal vein 4 h after MI model was established. Echocardiography test and Millar pressure-volume conductance catheter test were performed 7 d after MI model was established. Changes of cardiac function indexes of each group were recorded. Slices of cardiac tissue were stained by TTC and the area of MI was observed under optical microscope. Results  Results of Millar pressure-volume conductance catheter test showed that compared with the sham operation group, the cardiac output (CO), left ventricular end-systolic pressure (Pes), dP/dt_max, and left ventricular ejection fraction (LVEF) of the MI group remarkably decreased (P<0.05), while left ventricular end-diastolic pressure (Ped), dP/dt_min, and constant of isovolumetric relaxation (Tau) remarkably increased (P<0.05). CO, Pes, dP/dt_max, and EF of the BMMNCs group were higher than those of the MI group (P<0.05), while Ped, dP/dt_min, and Tau were significantly lower than those of the MI group (P<0.05). The differences of indexes between BMMNCs group and sham operation group were statistically significant (P<0.05). Echocardiography showed that interventricular septum of the MI group and BMMNCs group did not remarkably thicken. But compared with the sham operation group, the left ventricular diameter (LVD) and left ventricular end-diastolic/systolic volume (LVED, LVES) of the MI group and BMMNCs group increased (P<0.05). The observation of TTC stained cardiac tissue slices under optical microscope showed that the area of MI of the MMNCs group was significantly smaller than that of the MI group (P<0.05). Conclusion  Millar pressure-volume conductance catheter and echocardiography can monitor the cardiac function of mice from many aspects, evaluate the improvement of cardiac function by BMMNCs transplantation, and are significant for investigating the changes of cardiac function of mice after the incidence of MI.

Key words: Millar pressure-volume conductance catheter, echocardiography, myocardial infarction, bone marrow mononuclear cell transplantation