›› 2018, Vol. 38 ›› Issue (7): 845-.doi: 10.3969/j.issn.1674-8115.2018.07.025

• Techniques and methods • Previous Articles    

Condition optimization of the flow cytometry MoFlo Astrios EQ on single-cell sorting in 96-well plate

ZHANG Xiao-cui, FU Rong, ZHAO Ben-peng   

  1. Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
  • Online:2018-07-28 Published:2018-07-30

Abstract: Objective · To optimize the method of the flow cytometry MoFlo Astrios EQ on single-cell sorting in 96-well plate. Methods · Using different aperture nozzles and sorting ways, the 32D, U937, iBMDM and 293T cells were used for single-cell sorting after the precise adjustment of the instrument and various parameters. The hole numbers with single cell and single-cell clones were detected after sorting. Results · In the single-cell sorting mode, the hole numbers with single cell were 83-9170 μm nozzle and 87-93100 μm nozzle. After 7-10 days of culture, the hole numbers with single-cell clones were 36-5870 μm nozzle. In 100μmnozzle, the hole numbers with single-cell clones were53-78electrostatic charge sorting and 69-81straight-down sorting, respectively. Conclusion · In single-cell sorting, a better cell viability and higher cloning rate are observed in 100 μm nozzle and straight-down sorting.

Key words: flow cytometry, 96-well plate, single-cell sorting, nozzle, straight-down sorting, single-cell culture

CLC Number: