›› 2020, Vol. 40 ›› Issue (3): 317-.doi: 10.3969/j.issn.1674-8115.2020.03.007

• Original article (Basic research) • Previous Articles     Next Articles

Bioinformatics analysis of ulcerative colitis and its malignant complications and screening of potential therapeutic drugs

XIA Shou-bing, XU Chun-jie, JIANG Chun-hui, GU Lei, SUN Long-ci, XU Qing   

  1. Department of Gastrointestinal Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2020-03-28 Published:2020-04-09

Abstract: Objective · To explore the pathogenic genes and potential drug therapeutic targets of ulcerative colitis and its malignant complicationsa variety of bioinformatics analysis. Methods · Four profiling datasets (GSE13367, GSE9452 and GSE36807 as UC group, and GSE37283 as UCN group) downloaded the Gene Expression Omnibus (GEO) database were jointly analyzed to identify the differential genes. Key genes related to ulcerative colitis and its malignant complications were obtainedsubsequent immunoassay and gene correlation analysis, and a number of small molecule drugs with potential therapeutic effects were screenedLINCS L1000 database. Results · Eighty-six and 253 significant differentially expressed genes were identified respectively in the differential gene analysis of UC group and UCN group. The scoring analysis of the node genes in protein-protein interaction (PPI) network of UC group showed that the core gene of the network was CXCL8. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis indicated that CXCL8 mainly participated in the recruitment of neutrophils in IL-17 signaling pathway, and three small molecule drugs (butein, levocetirizine, pseudoephedrine) for CXCL8 were found based on the screening results of LINCS L1000 database. Conclusion · The core differentially expressed gene CXCL8 may be a new drug target for the treatment of ulcerative colitis and its malignant complications. The three screened small molecule drugs may have potential treatment effect on ulcerative colitis and its malignant complications.

Key words: ulcerative colitis, R language, bioinformatics, KEGG enrichment analysis, gene set enrichment analysis