Study on differentially expressed microRNA as a biomarker of polycystic ovary syndrome

doi:10.3969/j.issn.1674-8115.2021.11.005

Abstract

Abstract: Objective

·To explore microRNAs (miRNAs) as biomarkers of polycystic ovary syndrome (PCOS) and the biological significance in the pathogenesis of PCOS.

Methods

·Five patients with PCOS from January 2019 to October 2019 in Renji Hospital, Shanghai Jiao Tong University School of Medicine were selected as the observation group, and 5 healthy women were selected as the control group. Granulosa cells from 2 groups were collected and RNA was extracted by TRIzol method. TruSeq Small RNA Library Prep Kit was used to construct miRNA-Seq library. After the quality inspection of the library, NextSeq500 was used for high-throughput sequencing. Bioinformatics data analysis was performed on the sequencing results. RT-qPCR was used to verify the differential expression of candidate miRNA markers.

Results

·We finally got 20 differentially expressed miRNAs (all P<0.05). Among them, miR-196a-5p, miR-10a-5p and miR-451a were significantly up-regulated, while miR-877-5p and miR-2355-5p were significantly down-regulated. These differentially expressed miRNAs and their target genes may be involved in the transcriptional regulation mechanism of PCOS occurrence and development.

Conclusion

·Differentially expressed miRNAs have potential as PCOS biomarkers and are involved in the occurrence and development of PCOS.

Key words: microRNA (miRNA), biomarker, polycystic ovary syndrome, gene expression, target gene

CLC Number:

R711.75

Yu-huan WANG, Yi-cen DING, Yao-yu CAI, Ya-ni KANG. Study on differentially expressed microRNA as a biomarker of polycystic ovary syndrome[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(11): 1429-1435.

Figures/Tables 5

Tab 1 miRNA primer sequence（5′→3′）

Primer		Sequence 5′→3′
miRNA	hsa-miR-196a-5p	GGGTAGGTAGTTTCATGTT
	hsa-miR-10a-5p	GGGTACAGTATAGATGA
	hsa-miR-451a	GGGAAACCGTTACCATTAC
	hsa-miR-877-5p	GGGGTAGAGGAGATGGC
	hsa-miR-2355-5p	GGGATCCCCAGATACAAT
	Adaptor	GATCGTCGGACTGTAGAACT

Fig 1 Results of miRNA differential analysis

Fig 2 Results of functional enrichment analysis of target genes

Fig 3 RT-qPCR validation of 5 miRNA candidate markers

Fig 4 Transcriptional regulatory mechanism of PCOS occurrence and development

TrendMD

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