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Effects of Rhizoma Atractylodis before and after being fried with bran on immune system and gastrointestinal hormones of rats with experimental spleen deficiency

LIU Fen1,2, LIU Yan-ju2, TIAN Chun-man1   

  1. 1.Department of Chinese Medicine Teaching and Research Section， College of Traditional Chinese Medicine，Hubei Institute for Nationalities, Enshi 445000, China; 2.Department of Chinese Medicine Chemistry Teaching and Research Section， College of Pharmacy， College of Pharmacy of Hubei University of Chinese Medicine， Wuhan 430065, China
  • Online:2015-01-28 Published:2015-01-29
  • Supported by:

    National Natural Science Foundation of China,81073051; Scientific Research Special Project of Traditional Chinese Medicine Industry, State Administration of Traditional Chinese Medicine of China, 201007012-2; Independent Subject Research Project of Institute of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine of China, 2011ZDXK-02

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Abstract:

Objective To explore the effects of Rhizoma Atractylodis before and after being fried with bran on the gastrointestinal motility and immune function of rats with experimental spleen deficiency syndrome and to confirm if Rhizoma Atractylodis fried with bran can strengthen the effect of Rhizoma Atractylodes on interventing the spleen deficiency syndrome and possible mechanisms. Methods Rats were fed with Xiaochengqi Decoction combined with irregular hunger method to establish the spleen deficiency syndrome model. After the model was successfully copied, rats were randomly divided into the normal group, model group, Rhizome Atractylodes group, Rhizome Atractylodes fried with bran group, and domperidone group. After 10 d of treatment, the residual rate of stomach and small intestinal propulsion ratio of the rats were detected by the charcoal gavage method. The serum gastrin (GAS), motilin (MTL), and vasoactive intestinal peptide (VIP) levels were detected by the ELISA method. The serum interleukin (IL)-1, -2, -6 and tumor necrosis factor (TNF)-α levels were detected by the radioimmunoassaymethod. The indexes of spleen and thymus and the proliferation rate of spleen lymphocytes (T, B) were measured. Results Compared to the normal group, the gastrointestinal dynamics, hormone expressions, and immune function of the model group were significantly abnormal. Compared to the model group, residual rates of stomach of the Rhizome Atractylodes group, Rhizome Atractylodes fried with bran group, and domperidone group significantly decreased; small intestinal propulsion ratios significantly increased; serum GAS, MTL, and VIP levels increased with different degree; serum IL-1, -2, -6 and TNF-α levels also increased with different degree; and indexes of spleen and thymus and proliferation rates of spleen lymphocytes (T, B) increased with different degree and the differences were statistically significant (P<0.05 or P<0.01). Compared to the Rhizome Atractylodes group, the effects of Rhizome Atractylodes fried with bran were more significant and the differences of two groups were statistically significant (P<0.05 or P<0.01). Conclusion Rhizome Atractylodes extract can improve the gastrointestinal motility and regulate gastrointestinal hormone secretion and immune function of experimental spleen deficiency rats. The effects of Rhizome Atractylodes fried with bran are better than those of Atractylodes rhizome.

Key words: Rhizome Atractylodes extract, fried with bran, spleen deficiency syndrome, gastrointestinal hormone, immunity