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Preliminary study on transfection of human periodontal ligament cells and human bone marrow mesenchymal cells by human β-defensin 3

ZHU Jian-hua1, MIAO Bo1, LIU Na1,  WANG Hai-yan2   

  1. 1.Jiamusi Oral Medical Hospital, Jiamusi University, Jiamusi 154002,  China; 2.Department of Oral Mucosa Diseases, Shanghai Research Institute of Stomatology, Shanghai Key Lab of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology,  Shanghai Jiao Tong University School of Medicine, Shanghai 200011,  China
  • Online:2015-06-28 Published:2015-07-30
  • Supported by:

    National Natural Science Foundation of China, 81271157; State Key Clinical Specialty Construction Project of China, 2013-554

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Abstract:

Objective To investigate the transfection efficiency and bone formation of human periodontal ligament cells (hPDLCs) and human bone marrow mesenchymal cells (hBMCs) after being transfected by the lentivirus containing the gene of human β-defensin 3 (hBD3). Methods The lentivirus containing target gene and green fluorescent protein (GFP) gene was transfected to hPDLCs and hBMCs by the transfection agent polybrene. The expression of hBD3 was detected by the RT-PCR and Western blotting. The green fluorescence microscope was adopted to count cells and the transfection efficiency was calculated. The potential of bone formation was detected by the alkaline phosphatase staining and alizarin red staining. Results The transfection efficiencies of lentivirus containing hBD3 and GFP genes towards hBMCs and hPDLCs were 44.44% and 28.98%, respectively. The transfection efficiencies of lentivirus containing only GFP gene towards hBMCs and hPDLCs were 79.94% and 31.71%. Conclusion The transfection efficiencies of hPDLCs and hBMCs towards hBD3 gene are similar. And hPDLCs and hBMCs are ideal candidates for promoting the regeneration of periodontal tissue and transfected cells are still capable of forming bone.

Key words: human β-defensin 3, human periodontal ligament cells, human bone marrow mesenchymal cells, transfection, lentivirus