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Effects of miR-506 on the proliferation and metastasis in gastric cancer cells

LIN Zi-long1,2， ZHOU Quan2， REN Jia2， LI Jian-fang2， YU Bei-qin2， SU Li-ping2， XU Min-juan1， LIU Bing-ya1,2   

  1. 1.Shanghai Center for Systems Biomedicine, Ministry of Education Key Laboratory of Systems Biomedicine， Shanghai Jiao Tong University， Shanghai 200240， China； 2.Shanghai Key Laboratory of Gastric Neoplasms， Department of Surgery， Shanghai Institute of Digestive Surgery， Ruijin Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200025，China

  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Natural Science foundation of China， 91529302； National Key Technology Research and Development Program of the Ministry of Science and Technology of China， 2014BAI09B03

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Abstract:

Objective To investigate the effects of miR-506 on inhibiting the proliferation and metastasis in gastric cancer cells. Methods The miR-506 expressions in five gastric cancer cell lines (MKN-45, SGC-7901, BGC-823, NCI-N87, and AGS) were measured with real-time fluorescent quantitative PCR. The immortalized human normal gastric mucosa epithelial cell line GES-1 was served as control. MiR-506 mimics and anti-miR-506 were transiently transfected into SGC-7901 cell line to up-regulate and down-regulate the miR-506 expression with liposome transfection method. Changes in the proliferation of SGC-7901 were observed with CCK-8, while cell migration and invasion abilities were observed with wound healing assay and Transwell migration and invasion assay. Results The expressions of miR-506 in five gastric cancer cell lines were significantly lower as compared with normal gastric mucosa epithelial cell line GES-1 (P<0.05). MiR-506 over-expression could significantly inhibit the proliferation, migration, and invasion abilities in SGC-7901 cells. Compared with negative controls, the difference was statistically significant (P<0.01)．The inhibition of miR-506 expression could promote cell proliferation, migration, and invasion abilities (P<0.01). Conclusion The expression of miR-506 significantly decreases in gastric cancer cell lines. Up-regulation of miR-506 can inhibit proliferation, migration, and invasion abilities in gastric cancer cells, while down-regulation of miR-506 has the opposite effects.

Key words: miR-506； gastric cancer； proliferation, migration, invasion