Journal of Shanghai Jiao Tong University (Medical Science) ›› 2024, Vol. 44 ›› Issue (6): 702-712.doi: 10.3969/j.issn.1674-8115.2024.06.005
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TAN Lu1(), SHEN Shaoming2, HE Ping3(
)
Received:
2024-02-26
Accepted:
2024-03-15
Online:
2024-06-28
Published:
2024-06-28
Contact:
HE Ping
E-mail:tanlu98@sjtu.edu.cn;phe@shsci.org
Supported by:
CLC Number:
TAN Lu, SHEN Shaoming, HE Ping. Function and mechanism study of hypoxia-induced long non-coding RNA 68 in hepatocellular carcinoma[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2024, 44(6): 702-712.
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URL: https://xuebao.shsmu.edu.cn/EN/10.3969/j.issn.1674-8115.2024.06.005
Primer | Forword (5'→3') | Reverse (5'→3') |
---|---|---|
HILRNA44 | GGAACGAACTCTTGCGTGTG | AGGAGCGCACTTCCTCATTC |
HILRNA68 | AAGCGGAGCCTGAAGATGTG | CCAACCCGGCTGCTACTTTAT |
GLUT1 | CTGTCGTGTCGCTGTTTGTG | AAAGATGGCCACGATGCTCA |
PDK1 | CTGGCTGTGGCTTCTCTAGC | CCGAAGTCCAGGAACTGCTT |
PGK1 | TGTGGTCCTGAAAGCAGCAA | AGTTGACTTAGGGGCTGTGC |
BNIP3L | GGACTCGGCTTGTTGTGTTG | TCGACTAGGTGGGACGACAT |
VEGF | TAAGTCCTGGAGCGTTCCCT | ACGCGAGTCTGTGTTTTTGC |
18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
Tab 1 Primer sequences for qRT-PCR
Primer | Forword (5'→3') | Reverse (5'→3') |
---|---|---|
HILRNA44 | GGAACGAACTCTTGCGTGTG | AGGAGCGCACTTCCTCATTC |
HILRNA68 | AAGCGGAGCCTGAAGATGTG | CCAACCCGGCTGCTACTTTAT |
GLUT1 | CTGTCGTGTCGCTGTTTGTG | AAAGATGGCCACGATGCTCA |
PDK1 | CTGGCTGTGGCTTCTCTAGC | CCGAAGTCCAGGAACTGCTT |
PGK1 | TGTGGTCCTGAAAGCAGCAA | AGTTGACTTAGGGGCTGTGC |
BNIP3L | GGACTCGGCTTGTTGTGTTG | TCGACTAGGTGGGACGACAT |
VEGF | TAAGTCCTGGAGCGTTCCCT | ACGCGAGTCTGTGTTTTTGC |
18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
shRNA | Sequence (5'→3') |
---|---|
shHIF1α#1 | CCGCTGGAGACACAATCATAT |
shHIF1α#2 | GTGATGAAAGAATTACCGAAT |
shHIF1β#1 | GGCTCAAGGAGATCGTTTATT |
shHIF1β#2 | GCCTACACTCTCCAACACAAT |
shHIF2α | CAGTACCCAGACGGATTTCAA |
Tab 2 shRNA targeting sequences
shRNA | Sequence (5'→3') |
---|---|
shHIF1α#1 | CCGCTGGAGACACAATCATAT |
shHIF1α#2 | GTGATGAAAGAATTACCGAAT |
shHIF1β#1 | GGCTCAAGGAGATCGTTTATT |
shHIF1β#2 | GCCTACACTCTCCAACACAAT |
shHIF2α | CAGTACCCAGACGGATTTCAA |
siRNA | Sequence (5'→3') |
---|---|
siNC | TTGTTACTCGCTCACATTTAA |
si#1 | CGTACCTGGCCTTAGTAATTT |
si#2 | GAGCCTGAAGATGTGATTAAA |
Tab 3 siRNA targeting sequences
siRNA | Sequence (5'→3') |
---|---|
siNC | TTGTTACTCGCTCACATTTAA |
si#1 | CGTACCTGGCCTTAGTAATTT |
si#2 | GAGCCTGAAGATGTGATTAAA |
RNA | Coding probability | Label |
---|---|---|
HILRNA68 | 0.009 4 | Noncoding |
HIF1α | 1.000 0 | Coding |
XIST | 0.219 1 | Noncoding |
Tab 3 Coding potential analysis of HILRNA68
RNA | Coding probability | Label |
---|---|---|
HILRNA68 | 0.009 4 | Noncoding |
HIF1α | 1.000 0 | Coding |
XIST | 0.219 1 | Noncoding |
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