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Surrogate analyte-based quantitative measurement of the tyrosine level in cell culture media by HILIC-MS/MS

LI Wen-bin, DONG Jiang-kai*, MENG Shuang, LEI Hui-min, WANG Cong-hui, SHEN Ying, TANG Ya-bin, ZHU Liang   

  1. Department of Pharmacology, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China
  • Online:2016-07-28 Published:2016-08-31
  • Supported by:

    National Natural Science Foundation of China, 81173084, 81573018; Foundation of Shanghai Municipal Science and Technology Oommission, 15YF1406700

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Abstract:

Objective To non-invasively and dynamically monitor abnormal tyrosine metabolism in lung cancer cell lines by accurately measuring the tyrosine level in cell culture media. Methods The cell culture media samples were collected after cell lines 16HBE, PC9, and HCC827 were cultured for 24, 48, and 72 h. A HILIC-MS/MS method based on the surrogate analyte of tyrosine was developed to quantitatively measure the tyrosine level in cell culture media at different time points. Results This method showed a good linear correlation within the concentration range of 1-40 μg/mL (R=0.999 9) with relative recovery rates of 93.02%-102.53% and good repeatability (3.91%-8.69%). Conclusion This method is suitable for measuring the tyrosine level in cell culture media and can be used to observe specific tyrosine metabolism in lung cancer cell lines.

Key words: tyrosine, surrogate analyte, HILIC-MS/MS, cell culture media