›› 2019, Vol. 39 ›› Issue (4): 353-.doi: 10.3969/j.issn.1674-8115.2019.04.004

• Original article (Basic research) • Previous Articles     Next Articles

Generation and phenotypic analysis of Fbxo22knockout mice

ZHANG Hui-lin, ZHU Xiao-na, YANG Shuo, LIU Meng-di, ZHU Di, YU Yun   

  1. Center of Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-04-28 Published:2019-05-23
  • Supported by:
    National Natural Science Foundation of China, 81772936; Foundation of the International Peace Maternity & Child Health Hospital of China, GFY5809

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Abstract: Objective · To establish the Fbxo22 knockout momodel and study the biological function of FBXO22. Methods · The Fbxo22 knockout mice were generatedCRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/-mice were analyzed. Results · Although the Fbxo22-/-embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/- mice showed reduced body size and food intake and decreased life span. Conclusion · FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development. [Key words]FBXO22; CRISPR-Cas9 technology; early postnatal lethality

Key words: FBXO22, CRISPR-Cas9 technology, early postnatal lethality

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