Abstract: Objective · To establish the reference intervals of serum anti-Müllerian hormone (AMH) in healthy women of childbearing age in different age groups in Shanghai. Methods · A total of 239 healthy women of childbearing age, who accepted staff physical examination in International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, during July 2019, were enrolled and detected for serum AMH level using chemiluminescence immunoassay. After the outlier exclusion, 234 subjects were finally included and divided into six age groups. The reference intervals of serum AMH in healthy women of childbearing age in different age groups in Shanghai were established. The natural logarithm of serum AMH level and age were fitted by quadratic regression model. Results · The reference interval of serum AMH level was expressed as M(P5–P95), which were 4.76 (0.96–14.17) ng/mL in women aged 19–24 years, 6.03 (1.38–16.56) ng/mL in women aged 25–29 years, 4.30 (0.95–10.73) ng/mL in women aged 30–34 years, 2.40 (0.33–9.27) ng/mL in women aged 35–39 years, 1.04 (0.05–4.00) ng/mL in women aged 40–44 years, and 0.21 (0.01–3.04) ng/mL in women aged 45–49 years. After logarithmic transformation, serum AMH level and age could well obey the quadratic regression model (R2=0.502). Conclusion · The reference intervals of serum AMH level established for the first time in healthy women of child-bearing age in Shanghai may provide the basis for the evaluation of ovarian reserve function of women of different ages.

Key words: anti-Müllerian hormone (AMH), childbearing age, reference interval, Shanghai