JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (1): 89-94.doi: 10.3969/j.issn.1674-8115.2021.01.016

• Review • Previous Articles     Next Articles

Review of proteomic study of protein SUMOylation

Qiang-qiang XIONG1(), Jun TU2, Jun-ru LI2,3, Jin-ke CHENG2, Jian-hong ZUO1(), Ya-lan CHEN2()   

  1. 1.Research Lab of Translational Medicine, Hengyang Medical School, University of South China, Hengyang 421001, China
    2.Shanghai Key Laboratory for Tumor Microenvironment and Inflammation; Department of Biochemistry and Molecular Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
    3.Eight-year Specialty of Grade 2016 Clinical Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2021-01-28 Published:2021-02-22
  • Contact: Jian-hong ZUO,Ya-lan CHEN E-mail:1216268842@qq.com;632138414@qq.com;chenyl@shsmu.edu.cn
  • Supported by:
    Funding Information] National Natural Science Foundation of China(81700701);Innovative Research Team of High-Level Local University in Shanghai(SSMU-ZDCX20180802);Key Program of China Hunan Provincial Science & Technology Department(2017SK2082);Key Program of Health Commission of Hunan Province(20201909)

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Abstract:

Small ubiquitin-related modifier (SUMO) exists widely in all eukaryotic cells, which is involved in many signal transduction and metabolism pathways. Systematic identification of SUMOylated proteins is helpful to reveal the function and mechanism of SUMOylation from an overall perspective. At present, it is difficult to identify the SUMOylation sites, and how to study the SUMOylated proteins on a large scale has not been determined. This paper systematically analyzes the proteomic studies of protein SUMOylation in mammalian cells. On the basis of summarizing the research methods and achievements, the mutation mode and enrichment mode of SUMO are analyzed in detail, and then the ideal methods for systematic identification of SUMOylation sites in cells and tissues are concluded.

Key words: small ubiquitin-related modifier (SUMO), SUMOylation, proteomics, mass spectrometry

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