Abstract:

Objective To investigate the association of single nucleotide polymorphisms (SNPs) in regulated upon activation normal T cell expressed and secreted (RANTES) gene C-28G (RANTES C-28G), RANTES A-403G and Eotaxin-3 gene C+77T (Eotaxin-3 C+77T) with asthma in Han ethnic children. Methods The buccal mucosa swabs of 192 Han ethnic children with asthma (asthma group) were collected,  and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect the SNP loci of RANTES A-403G, C-28G and Eotaxin-3 C+77T. Besides, another 192 healthy subjects (aged 18 to 22 years) without sibship with those in asthma group were served as controls. Genotype and genotypic distribution between these two groups were analysed. Results There was no significant differences in genotype and genotypic distribution of SNP loci of RANTES A-403G and RANTES C-28G between asthma group and control group (P>0.05), while there were significant differences in genotypic distribution of Eotaxin-3 C+77T between these two groups. The frequency of Eotaxin-3 C+77T T/T genotype in asthma group was significantly higher than that in control group (32.3% vs 12.5%, OR=3.44, P=0.000). Conclusion Eotaxin-3 C+77T may be the asthma susceptible SNP loci for Han ethnic children, and Eotaxin-3 C+77T T/T is significantly related with the development of childhood asthma.

Key words: regulated upon activation normal T cell expressed and secreted, Eotaxin, single nucleotide polymorphisms, asthma, children, Han ethnic