›› 2010, Vol. 30 ›› Issue (9): 1084-.doi: 10.3969/j.issn.1674-8115.2010.09.017

• Original article (Basic research) • Previous Articles     Next Articles

Effects of IL-1 beta and TNF-alpha on degradation of extracellular matrix of articular chondrocytes and related mechanism

HUANG Jin-gang1, TONG Hai-jun1, LIU Hong-qiang2, ZHANG Xiao-ling1,3   

  1. 1.Laboratory of Orthopedical Cellular and Molecular Biology, Institute of Health Sciences, Shanghai Jiaotong University School of Medicine &|Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China；2.College of Physical Education, Shanxi University, Taiyuan 030006, China；3.Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedics, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2010-09-25 Published:2010-09-27
  • Supported by:

    National Natural Science Foundation of China, 30811120440；Shanghai Science and Technology Committee Foundation, 08410701800, 08411950400；Shanghai Education Committee Foundation, J50206

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Abstract:

Objective To investigate the effects of interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) on the degradation of extracellular matrix of articular chondrocytes, and explore the related molecular mechanism. Methods Primary articular chondrocytes were isolated from rat articular chondrocytes, and were stimulated by IL-1β and TNF-α alone or in combination. IL-1β stimulation group, TNF-α stimulation group and IL-1β and TNF-α stimulation group were established, and control group without any stimulation was also established. After treatment for 24 h, 48 h and 72 h, the changes of extracellular matrix of articular chondrocytes were observed by inverted microscope, and Real-time PCR was employed to detect the expression of metalloproteinase-13 (MMP-13), Aggrecanases-1 (Adamts-4) and Aggrecanases-2 (Adamts-5) mRNA. Results After treatment for 48 h and 72 h, it was observed by microscope that there was no significant difference in degradation of extracellular matrix of articular chondrocytes between control group and TNF-α stimulation group, while extracellular matrix of articular chondrocytes in IL-1β stimulation group and IL-1β and TNF-α stimulation group was unstained and degraded with expanded cell spaces. Compared with control group, the expression of MMP-13, Adamts-4 and Adamts-5 mRNA in IL-1β stimulation group significantly increased after treatment for 24 h （P<0.01）, and decreased after treatment for 48 h and 72 h. There was no significant difference in the expression of MMP-13, Adamts-4 and Adamts-5 mRNA between IL-1β stimulation group and IL-1β and TNF-α stimulation group after treatment for 24 h, 48 h and 72 h （P>0.05）. Conclusion IL-1β can directly degrade extracellular matrix of articular chondrocytes through up-regulation of expression of MMP-13, Adamts-4 and Adamts-5, while TNF-α can not directly degrade extracellular matrix of articular chondrocytes.

Key words: osteoarthritis, metalloproteinase, aggrecanase, interleukin-1β, tumour necrosis factor-α