Abstract:

Objective To establish the suitable animal model for pathological study of venous hypertension, and investigate the role of Duffy antigen receptor for chemokines (DARC) in capillary endothelial cells in the inflammatory reaction of venous hypertension. Methods SD rat femoral arteriovenous fistula venous hypertension model and iliac vein coarctation venous hypertension model were established, and negative controls and blank controls were also prepared. The changes of area of blood capillary lumens in muscle and subcutaneous tissues in hind limbs of rats were observed by HE staining and immunohistochemical staining, and the changes of expression of DARC in capillary endothelial cells were detected with selected rat models. Results The area of blood capillary lumens in muscle and subcutaneous tissues in hind limbs of rat femoral arteriovenous fistula venous hypertension model was much larger and more sustained than that of rat iliac vein coarctation venous hypertension model at different time points after model establishment. The expression of DARC in capillary endothelial cells of rat femoral arteriovenous fistula venous hypertension model increased with time after model establishment. Conclusion Rat femoral arteriovenous fistula model is more suitable for pathological study of venous hypertension, and the expression of DARC in capillary endothelial cells is related with inflammatory reaction of venous hypertension.

Key words: Duffy antigen receptor for chemokines, venous hypertension, animal model, chronic venous insufficiency