›› 2010, Vol. 30 ›› Issue (10): 1208-.doi: 10.3969/j.issn.1674-8115.2010.10.006

• Original article (Basic research) • Previous Articles     Next Articles

Effects of hypoxia culture on damage and apoptosis of RL95-2 cell line

XU Bu-fang1, SUN Xiao-xi2, |FENG Yun1, |ZHANG Ai-jun1, CHENG Li-nan3   

  1. 1.Reproduction Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；2.Shanghai |JiAi Genetics and IVF Institute, Shanghai 200023, China；3.Institute of Family Planning, Shanghai Medical School, Fudan University, Shanghai 200032, China
  • Online:2010-10-25 Published:2010-10-27
  • Supported by:

    Science and Technology Foundation of Shanghai Jiaotong University School of Medicine, YZ1041

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Abstract:

Objective To investigate effects of hypoxia culture on mitochondria damage and apoptosis of human endometrial epithelial carcinoma cell line (RL95-2). Methods RL95-2 cells were cultured with medium containing different concentrations of CoCl2 (0, 10, 30, 62.5, and 125 μmol/L). Cell proliferation activity, mitochondria membrane potential, and apoptosis rate were determined via CCK-8 kit, JC-1 fluorescence staining with flow cytometry, and Annex V/PI. Results With CoCl2 concentration rising or extension of action time (62.5 μmol/L), the proliferation activity of RL95-2 cells was weak, while the depolarization of mitochondria membrane and the apoptosis rate were increased. Conclusion The condition of hypoxia induces decrease of proliferation activity, mitochondria damage, increase of apoptosis of endometrial epithelial cells.

Key words: hypoxia culture, RL95-2 cell line, mitochondria damage, apoptosis