Abstract:

Objective To investigate the effects of deuterium-depleted Chinese liquor on fasting blood glucose (FBG) and islet cells and their function of diabetic rats. Methods Wister rat diabetic models were established by streptozotocin (STZ) one-time intraperitoneal injection. The experimental rats were divided into following groups (n=10) according to the means of intervention: model group (intragastric administration of normal saline, fed with normal water), deuteriumdepleted water (DDW) group (intragastric administration of DDW, fed with DDW), normal Chinese liquor group\[intragastric administration of one unit (low) or six unit (high) Chinese liquor and distilled water, fed with normal water\], deuterium-depleted Chinese liquor group (intragastric administration of low or high dose of Chinese liquor and DDW, fed with DDW), and control group (without STZ injection, intragastric administration of normal water, fed with normal water). An oral daily administration of liquor solution was 0.01 mL/g (volume/body weight). At the end of 4 weeks of intervention, the blood was collected from the orbital vein followed by killing the rats with the method of cervical dislocation. Then, pancreatic tissues were removed to make samples. The FBG was measured by glucose oxidase, and the fasting insulin levels (FINS) were analyzed by radioimmunoassay. The pathology structure of pancreatic islets was observed by HE staining. Results FBG in every group followed the order: normal Chinese liquor (high) group>model group>normal Chinese (low) liquor group>deuterium-depleted Chinese liquor (high) group>DDW group>deuterium-depleted Chinese liquor (low) group. And FBG of DDW group and deuteriumdepleted Chinese liquor (low) group had significant difference to other groups (P<0.05). FINS of every group followed the order: deuterium-depleted Chinese liquor (low) group>deuterium-depleted Chinese liquor (high) group>normal Chinese liquor (low) group>DDW group>normal Chinese liquor (high) group>model group. And FINS of other groups had significant differences to model group （P<0.05）except for the normal Chinese liquor (high) group. The observation of histological examination revealed that compared to control group, in model group and normal Chinese liquor group, the cell amount of pancreatic islets decreased and coupled with volume increase, vacuolar cytoplast, and disorder. While in DDW and deuterium-depleted Chinese liquor groups, pancreatic islet cells had mild damage. Conclusion The deuterium-depleted Chinese liquor has significant effects in decreasing FBG of rats, increasing FINS, and reduces the islet cells damage caused by excessive drinking.

Key words: deuterium-depleted water, Chinese liquor, diabetes mellitus, fasting blood glucose, fasting insulin levels, islet cell