›› 2012, Vol. 32 ›› Issue (1): 9-.doi: 10.3969/j.issn.1674-8115.2012.01.002

• Original article (Basic research) • Previous Articles     Next Articles

Responsive ability of rats with type 2 diabetes under acute inflammatory state

HAN Ting-ting1, SU Budegerile1, HU Yao-min1, HUANG Rong1, YI Jing2   

  1. 1.Department of Endocrinology and Metabolic Diseases, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China；2.Department of Cell Biology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2012-01-28 Published:2012-01-29
  • Supported by:

    National Natural Science Foundation of China, 81170758, 30670988；Foundation from Renji Hospital, RJPY10-004；Scientific Research Foundation for Excellent Young Teachers in Shanghai, jdy09018

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Abstract:

Objective To investigate the differences of responsive ability and blood glucose between rats with type 2 diabetes and normal rats under acute inflammatory state. Methods GK rats with type 2 diabetes and Wistar rats (normal controls) were selected, and critical ill models of sepsis were established by intraperitoneal injection of 5 mg/kg lipopolysaccharide (LPS). Before and after LPS injection, the general state was observed, serum mass concentrations of pro-inflammatory cytokines of tumor necrosis factor α (TNF-α), interleukin 1β(IL-1β) and interleukin 6 (IL-6) and anti-inflammatory cytokine of interleukin 10 (IL-10) were measured, and blood glucose fluctuation was monitored in Wistar rats and GK rats. Results After LPS injection, the general state of Wistar rats was poor, and symptoms such as reduced activity, drowsiness, hair erection and severe diarrhea emerged. However, GK rats stayed in good condition, and did not bear the above symptoms. Before LPS injection, the concentration of blood glucose of GK rats was significantly higher than that of Wistar rats. After LPS injection, the concentrations of blood glucose of Wistar rats and GK rats significantly increased, the range of blood glucose fluctuation of GK rats was smaller than that of Wistar rats, while there was no significant difference in the peak concentration of blood glucose between GK rats and Wistar rats. Before LPS injection, serum mass concentrations of TNF-α and IL-6 of GK rats were significantly higher than those of Wistar rats （P<0.01）. The serum mass concentrations of TNF-α, IL-1β, IL-6 and IL-10 after LPS injection were significantly higher than those before LPS injection in GK rats and Wistar rats （P<0.01）, and the mass concentrations of TNF-α, IL-1β and IL-10 in Wistar rats were significantly higher than those in GK rats after LPS injection （P<0.01）. Conclusion The tolerance to acute inflammation of rats with type 2 diabetes is higher than that of normal rats.

Key words: diabetes mellitus, sepsis, acute inflammation, blood glucose, cytokine