Abstract:

Objective To investigate the value of domestic plasma (1-3)-β-D-glucan detection reagent in the diagnosis of invasive fungal disease (IFD). Methods Patients suspected of IFD were screened from Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine from April 2009 to June 2011. Based on the diagnostic standard of IFD from the European Organization for Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG), 63 true positive cases (57 confirmed as IFD and 6 preliminarily diagnosed as IFD) were selected as IFD group, and the other 100 nonIFD true negative cases were served as control group. Plasma mass concentrations of (1-3)-β-D-glucan were measured with domestic plasma (1-3)-β-D-glucan detection reagent, and comparison was made between groups. Receiver operating characteristic (ROC) curve was drafted, and the cutoff value of plasma (1-3)-β-D-glucan in diagnosis of IFD was analysed. Results Plasma levels of (1-3)-β-D-glucan in both groups exhibited non-normal distribution(positive skewness), with coefficient of kurtosis＞0 and coefficient of skewness＞0. The plasma mass concentrations of (1-3)-β-D-glucan of control group and IFD group were (33.62±5.0) pg/mL and (373.82±125.5) pg/mL respectively, and there were significant differences between them (P<0.001). Analysis of ROC curve revealed that the cutoff value of plasma (1-3)-β-D-glucan in diagnosis of IFD was 20.85, maximum area under the curve was 0.857 (P<0.05), 95% CI was 0.792 to 0.923, and values of sensitivity and specificity were 77.8% and 86.0% respectively. Conclusion Domestic plasma (1-3)-β-D-glucan detection reagent can be used in the diagnosis of IFD.

Key words: (1-3)-β-D-glucan, invasive fungal disease, diagnosis