上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (5): 594-.doi: 10.3969/j.issn.1674-8115.2012.05.013

• 论著（基础研究） • 上一篇    下一篇

聚氨酯复合材料表面变形链球菌黏附状况的实验研究

李 静, 孔方圆, 郑元俐   

  1. 上海交通大学 医学院附属第九人民医院口腔修复科 |上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2012-05-28 发布日期:2012-06-01
  • 通讯作者: 郑元俐, 电子信箱: zhengyuanli@yahoo.com.cn。
  • 作者简介:李 静（1974—）, 女, 主治医师, 博士；电子信箱: lijingpenglai@yahoo.com。
  • 基金资助:

    上海市科委资助项目（09411954700, 08DZ2271100）；上海市重点学科建设项目（S30206, T0202）

Study of Streptococcus mutans adhesion to polyurethane composites

LI Jing, KONG Fang-yuan, ZHENG Yuan-li   

  1. Department of Prosthodontics, the Ninth People's Hospital, College of Stomatology, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Online:2012-05-28 Published:2012-06-01
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 09411954700, 08DZ2271100；Shanghai Leading Academic Discipline Project, S30206, T0202

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摘要：

目的 观察含纳米聚磷腈的氨酯复合材料表面变形链球菌的黏附情况。方法 室温下分别固化含1%和5%纳米聚磷腈的氨酯复合材料（PZS-1%和PZS-5%组）和硅橡胶软衬材料（对照组），经试件准备和变形链球菌菌液制备后行黏附试验。厌氧培养48 h后，光学显微镜下观察经革兰染色试件表面的细菌黏附情况；经超声和漩涡震荡后，试件表面黏附的细菌脱落，收集菌悬液行变形链球菌菌落计数。结果 光学显微镜观察发现，3组试件表面黏附的变形链球菌形态相近，呈典型链状排列的革兰阳性球菌。3组试件表面黏附的变形链球菌菌落形成数与相应吸光度值具有较好的一致性。PZS-1%组、PZS-5%组和对照组试件表面变形链球菌菌落形成数分别为（405.000±60.046）、（253.000±62.946）和（1 008.000±119.955）（×102CFU/mL），PZS-5%组显著低于PZS-1%组和对照组，差异均有统计学意义（P<0.001）。结论 含5%纳米聚磷腈的氨酯复合材料表面的变形链球菌黏附数量较少，可能与其添加的聚磷腈成分有关。

关键词: 聚氨酯, 聚磷腈, 软衬, 变形链球菌, 黏附

Abstract:

Objective To investigate the Streptococcus mutans adhesion to polyurethane composites containing polyphosphazene. Methods Polyurethane composites containing 1% polyphosphazene (PZS-1% group), polyurethane composites containing 5% polyphosphazene (PZS-5% group) and silicone rubber soft liner (control group) were solidified under room temperature. These specimens were incubated with Streptococcus mutans suspension. Forty-eight hours after anaerobic culture, Streptococcus mutans adhesion to specimens was observed by light microscopy with Gram staining. Suspension with bacteria detached from specimens after ultrasound and vortex vibration was collected, and colonies of Streptococcus mutans were counted. Results Light microscopy revealed that the morphology of Streptococcus mutans adhered to specimens in three groups was similar, and exhibited typical chain arrangement of Gram positive coccus. The numbers of colonies of Streptococcus mutans adhered to specimens in three groups were well in line with corresponding absorbance. The numbers of colonies of Streptococcus mutans adhered to specimens in PZS-1% group, PZS-5% group and control group were (405.000±60.046), (253.000±62.946) and (1 008.000±119.955)(×102 CFU/m) accordingly, and the number of PZS-5% group was significantly lower than those of PZS-1% group and control group (P<0.001). Conclusion Relatively less Streptococcus mutans is adhered to polyurethane composites containing 5% polyphosphazene, which may be related to the addition of polyphosphazene.

Key words: polyurethane, polyphosphazene, soft liner, Streptococcus, adhesion