上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (10): 1338-.doi: 10.3969/j.issn.1674-8115.2012.10.013

• 论著（临床研究） • 上一篇    下一篇

甲氧西林耐药的CO2依赖型金黄色葡萄球菌小菌落突变株的分离和鉴定

俞 静, 刘 瑛, 陈 峰   

  1. 上海交通大学 医学院附属新华医院检验科, 上海 200092
  • 出版日期:2012-10-28 发布日期:2012-11-05
  • 通讯作者: 刘 瑛, 电子信箱: liuying0129@yahoo.com.cn。
  • 作者简介:俞 静(1982—), 女, 博士；电子信箱: fissh19@gmail.com。

Isolation and identification of CO2-dependent Staphylococcus aureus small colony variant with methicillin resistance

YU Jing, LIU Ying, CHEN Feng   

  1. Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2012-10-28 Published:2012-11-05

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摘要：

目的 对由临床分离的一株拟是金黄色葡萄球菌小菌落突变株（SCVs）进行鉴定、药敏试验和补偿试验，为预防和控制SCVs感染提供依据。方法 从患儿脑瘤切除部位分泌的脓液中分离培养出一株金黄色葡萄球菌，进行菌落形态观察及生化鉴定、药敏试验、金黄色葡萄球菌种特异性基因(nuc)和甲氧西林耐药相关基因(mecA)的测序以及补偿试验。结果 该菌株通过Vitek-2 Compact系统鉴定为金黄色葡萄球菌，该菌含有金黄色葡萄球菌菌种特异性基因nuc和甲氧西林耐药相关基因mecA。菌落形态主要表现为菌落细小、生长缓慢、溶血圈不明显（24 h）；凝固酶活性下降；触酶阳性；革兰染色为革兰阳性球菌，呈葡萄状排列；对β-内酰胺类抗生素有抗药性；补偿试验鉴定该SCVs为CO2依赖型。结论 成功分离鉴定出一株甲氧西林耐药的CO2依赖型金黄色葡萄球菌SCVs，为开展金黄色葡萄球菌SCVs相关疾病的预防和控制及其相关致病机制研究奠定基础。

关键词: 金黄色葡萄球菌, 小菌落突变株, 分离鉴定, CO2依赖型

Abstract:

Objective To identify a clinical isolate of Staphylococcus aureus small colony variant and investigate its antibiotic resistance and auxotroph type, so as to provide a basis for the prevention and control of small colony variant infection. Methods One possible Staphylococcus aureus small colony variant strain was isolated from pus of brain tumor resection site of a patient. The bacterial colony morphology, biochemical characteristics and susceptibility test of this small colony variant strain were identified. Staphylococcus aureus specific gene nuc and methicillin resistance-associated gene mecA were detected and sequenced. Complementation test was used to identify the type of auxotroph. Results The strain was identified as Staphylococcus aureus by Vitek-2 Compact system, which contained Staphylococcus aureus specific gene nuc and methicillin resistance-associated gene mecA. Its major phenotypes included small colony, slow growth, indistinct zone of hemolysis (24 h), decreased coagulase and positive catalase reaction. This clinical isolate of Gram positive cocci in grape like clusters was resistant to β-lactam antibiotics, and complementation test showed that the growth of this clone was CO2dependent. Conclusion One CO2-dependent methicillin resistant Staphylococcus aureus small colony variant strain was isolated and identified, which laid the groundwork for the control and prevention of Staphylococcus aureus small colony variant strain-associated disease and research of its pathogenic mechanism.

Key words: Staphylococcus aureus, small colony variants, isolation and identification, CO2-dependent