上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (2): 145-.doi: 10.3969/j.issn.1674-8115.2013.02.004

• 论著（基础研究） • 上一篇    下一篇

GX-50抗皮肤衰老的研究

侯雪峰1, 宋小飞2, 王朝霞1, 戴靖波1, 唐茂萍1, 陈晓辉1, 宋肖静3, 高 萍1, 乔中东1   

  1. 1.上海交通大学 生命科学技术学院, 上海 200240； 2.上海瑞金集团闵行中心医院泌尿外科, 上海 201100； 3.山西医科大学细胞生物学教研室, 太原 030001
  • 出版日期:2013-02-28 发布日期:2013-03-07
  • 通讯作者: 王朝霞, 电子信箱: zhaoxiaw@sjtu.edu.cn。
  • 作者简介:侯雪峰（1989—）, 女, 硕士生； 电子信箱: xueyuefenghua@sjtu.edu.cn； 宋小飞（1982—）, 男, 硕士； 电子信箱: sxfonline@qq.com。
  • 基金资助:

    江苏省无锡530计划（2011JDZX020）

Anti-skin aging effect of GX-50

HOU Xue-feng1, SONG Xiao-fei2, WANG Zhao-xia1, DAI Jing-bo1, TANG Mao-ping1, CHEN Xiao-hui1, SONG Xiao-jing3, GAO Ping1, QIAO Zhong-dong1   

  1. 1.School of Life Sciences and Technology, Shanghai Jiaotong University, Shanghai 200240, China； 2.Department of Urology, Minhang Central Hospital of Ruijin Group, Shanghai 201100, China；3.Department of Cytology, Shanxi Medical University, Taiyuan 030001, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    530 Project of Wuxi, Jiangsu Province, 2011JDZX020

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摘要：

目的 研究GX-50抗皮肤衰老的作用。方法 通过在20月高龄小鼠背部涂抹GX-50，采用HE染色和免疫组织化学法对GX-50抗皮肤衰老的作用进行组织学观察。原代培养人皮肤成纤维细胞，采用H2O2诱导建立皮肤细胞衰老模型，分为空白对照组、DMSO组、GX-50组、H2O2组和GX-50预处理后H2O2处理组（GX-50+H2O2组）；采用β-半乳糖苷酶染色实验以及测定细胞分泌物丙二醛（MDA）和细胞内活性氧（ROS）含量检测GX-50在细胞水平的抗衰老能力。应用Draize兔眼刺激性实验评价GX-50的刺激性。结果 在组织学水平，GX-50可使高龄小鼠衰老的皮肤年轻化，增加胶原蛋白的表达。在细胞水平，GX-50可使人皮肤成纤维细胞β-半乳糖苷酶合成降低；GX-50组细胞MDA分泌量较空白对照组下降30.45%，GX-50+H2O2组较H2O2组下降61.46%，组间比较差异均有统计学意义（P<0.05）；GX-50组细胞胞内ROS荧光值较空白对照组下降8.03%，GX-50+H2O2组较H2O2组下降8.36%，组间比较差异均有统计学意义（P<0.05）。Draize兔眼刺激性评分为0。结论 GX-50可以在一定程度上保护和延缓皮肤衰老，具有作为护肤品有效添加剂的应用前景。

关键词: GX-50, 皮肤衰老, 胶原蛋白, 丙二醛, 活性氧

Abstract:

Objective To investigate the protective effect of GX-50 against skin aging. Methods GX-50 was applied on the back of mice aged 20 months, and the effect of GX-50 on mouse skin was observed with HE staining and immunohistochemistry. Human skin fibroblasts were primarily cultured, senescent cell model was established by H2O2 induction, and blank control group, DMSO group, GX-50 group, H2O2 group, and GX-50+H2O2 group (pretreatment with GX-50+H2O2 treatment) were divided. The anti-aging effect of GX-50 on primary human skin fibroblasts was examined by SA-βGal staining, as well as determination of secreted malondialdehyde (MDA) concentration and intracellular reactive oxygen species (ROS) content. The irritation characteristics of GX-50 were detected by Draize eye test. Results The histological observation revealed that the GX-50 treated skin exhibited better appearance and higher collagen content. β-galactosidase in human skin fibroblasts was reduced by GX-50. Compared with blank control group, the secretion of MDA was down-regulated by 30.45% and the fluorescence value of ROS was down-regulated by 8.03% in GX-50 group (P<0.05). Compared with H2O2 group, the secretion of MDA was down-regulated by 61.46% and the fluorescence value of ROS was down-regulated by 8.36% in GX-50+H2O2 group (P<0.05). Draize eye test showed the irritation score of GX-50 was 0. Conclusion GX-50 has protective and anti-aging effect on skin, and may serve as a potential cosmetic additive against skin aging.

Key words: GX-50, skin aging, collagen, malondialdehyde, reactive oxygen species