›› 2010, Vol. 30 ›› Issue (2): 240-.

• 技术与方法 • 上一篇    下一篇

血管紧张素转化酶底物的制备及对肺癌诊断的临床意义

周海燕1, 姚莉韵2, 李盈懿3, 张建华2   

  1. 上海交通大学 1. 胸科医院检验科, 上海 200030, 2. 基础医学院化学教研室, 上海 200025, 3. 医学院辅导员办公室, 上海 200025
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 姚莉韵, 电子信箱: yaoliyun517@yahoo.com.cn。
  • 作者简介:周海燕(1970—), 女, 检验师;电子信箱: zhouhy180@sina.com。
  • 基金资助:

    上海市卫生局科技发展基金项目 (2007085)

Synthesis of substrate for angiotensin-converting enzyme and its value in diagnosis of lung cancer

ZHOU Hai-yan1, YAO Li-yun2, LI Ying-yi3, ZHANG Jian-hua2   

  1. 1. Department of Clinical Laboratory, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China;2. Department of Chemistry, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China;3. Office of Counselor, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007085

摘要:

目的 建立血管紧张素转化酶(ACE)底物的合成方法,探讨血清ACE活性的测定方法。方法 底物制备采用N-羟基琥珀酰亚胺活化酯合成多肽法;建立分光光度法测定ACE的活性。结果 底物呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸纯度为99.5%。底物的米氏常数Km为0.25 mmol/L,血清ACE活性正常参考值范围46.7~113.3 U/L,标本批内和批间平均变异系数分别为3.9%和4.9%。临床检测表明,肺鳞癌、肺腺癌患者血清ACE活性显著下降(P<0.05);而肺小细胞癌患者ACE活性没有明显降低(P>0.05)。结论 所合成的底物适用于临床诊断各类肺癌的要求。

关键词: 血管紧张素转化酶, 呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸, 肺癌

Abstract:

Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme (ACE), and investigate the assay method of serum ACE. Methods The substrate was prepared by the use of N-hydroxysuccinimide ester in peptide synthesis, and serum ACE was determined by spectrophotometric assay using substrate. Results The substrate of furanacryloyl-phenylalanyl-glycyl-glycine with purity of 99.5% was obtained. The Km value for the substrate was 0.25 mmol/L, the normal reference interval for serum ACE activity was 46.7 U/L to 113.3 U/L, and the within-run and between-run coefficient of variation (CVs) ranged from 3.9 to 4.9%. The serum ACE activity in patients with squamous carcinoma and adenocarcinoma of lung were significantly decreased (P<0.05), while that in patients with lung small cell carcinoma was not significantly decreased (P>0.05). Conclusion The synthesized substrate could be reliably used as a marker for diagnosis of different lung cancers in clinics.

Key words: angiotensin converting enzyme, furanacryloyl-phenylalanyl-glycyl-glycine, lung cancer