›› 2010, Vol. 30 ›› Issue (7): 802-.

• 论著(基础研究) • 上一篇    下一篇

高压氧对液压颅脑损伤大鼠脑组织线粒体及认知功能的影响

周正文, 王 勇, 樊翊凌, 万杰清   

  1. 上海交通大学 |医学院仁济医院神经外科, 上海 200127
  • 出版日期:2010-07-25 发布日期:2010-07-26
  • 通讯作者: 王 勇, 电子信箱: wangyong1960@126.com。
  • 作者简介:周正文(1967—), 男, 主治医师, 学士;电子信箱: zzwn2003@yahoo.com.cn。

Effects of hyperbaric oxygen on mitochondrial function of brain tissues and cognitive function after fluid-percussion injury in rats

ZHOU Zheng-wen, WANG Yong, FAN Yi-ling, WAN Jie-qing   

  1. Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
  • Online:2010-07-25 Published:2010-07-26

摘要:

目的 探讨高压氧(HBO)治疗对液压颅脑损伤大鼠脑组织线粒体和认知功能的改善作用。方法 SD大鼠分为模型组和假损伤组,其中模型组均经液压颅脑损伤建模并根据所接受的氧疗方式分为低浓度氧治疗组(n=23)、高浓度氧治疗组(n=24)和HBO治疗组(n=23);假损伤组(n=22)则接受与模型组同样的手术,但不予液压颅脑损伤,接受与低浓度氧治疗组相同的治疗。氧疗结束后,高效液相色谱分析测定大脑皮层三磷酸腺苷(ATP)水平;流式细胞仪检测脑组织线粒体活性氧分子(ROS)的生成;Nissl染色对海马区神经元进行计数。Morris水迷宫法记录大鼠逃避潜伏期,评判颅脑损伤后11~15 d的大鼠认知功能。结果 HBO治疗组和高浓度氧治疗组脑组织ATP水平与假损伤组接近(P>0.05),显著高于低浓度氧治疗组(P<0.05)。HBO治疗组海马CA2/3区和门区神经元计数均显著高于低浓度氧治疗组和高浓度氧治疗组(P<0.05)。各组间脑组织线粒体ROS生成量比较差异均无统计学意义(P>0.05)。Morris水迷宫测试中,HBO治疗组大鼠逃避潜伏期明显短于低浓度氧治疗组和高浓度氧治疗组(P<0.05)。结论 HBO治疗液压颅脑损伤大鼠,有助于维持大脑线粒体ATP合成功能;同时减少海马区神经元丢失,改善认知功能。

关键词: 颅脑损伤, 高压氧, 三磷酸腺苷, 海马神经元, 认知功能

Abstract:

Objective To investigate the effects of hyperbaric oxygen (HBO) on mitochondrial function of brain tissues and cognitive function after fluid-percussion injury in rats. Methods SD rats were divided into model group and sham injury group, and fluid-percussion injury was achieved in each rat of model group, which were subdivided into low concentration O2 treatment group (n=23), high concentration O2 treatment group (n=24) and HBO treatment group (n=23) according to different ways of O2 treatment. Rats in sham injury group (n=22) received the same operations with model group, while did not experience fluid-percussion injury, and were treated as low concentration O2 treatment group. After O2 treatment, high performance liquid chromatography was employed to detect cortical adenosine triphosphate (ATP) levels, flow cytometry was used to determine mitochondrial reactive oxygen species (ROS) production of brain tissues, and hippocampal neuron counting was performed with Nissl staining. Morris water maze test was adopted to record escape latency, and cognitive function 11 to 15 d after brain injury was evaluated. Results ATP levels of brain tissues in HBO treatment group and high concentration O2 treatment group were similar to that in sham injury group (P>0.05), and were significantly higher than that of low concentration O2 treatment group (P<0.05).  Hippocampal neuron counting in CA2/3 and hilus regions in HBO treatment group was significantly higher than that in low concentration O2 treatment group and high concentration O2 treatment group (P<0.05). There was no significant difference in mitochondrial ROS production of brain tissues among groups (P>0.05). In Morris water maze test, the escape latency of HBO treatment group was significantly shorter than those of low concentration O2 treatment group and high concentration O2 treatment group (P<0.05). Conclusion HBO can maintain brain mitochondrial ATP synthesis for rats with fluidpercussion injury.  Moreover, HBO can improve cognitive function and reduce hippocampal neuron loss after injury.

Key words: traumatic brain injury, hyperbaric oxygen, adenosine triphosphate, hippocampal neuron, cognitive function