上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (1): 39-.doi: 10.3969/j.issn.1674-8115.2011.01.009

• 论著（临床研究） • 上一篇    下一篇

系统性红斑狼疮患者外周血细胞miR-31的表达

范 薇1, 唐元家2, 曲 波2, 崔慧娟2, 黄新芳1, 陈顺乐1, 沈 南1,2   

  1. 1.上海交通大学医学院附属仁济医院风湿科 上海市风湿病学研究所, 上海 200001；2.中国科学院上海生命科学研究院/上海交通大学医学院健康科学研究所分子风湿病学研究组, 上海 200025
  • 出版日期:2011-01-28 发布日期:2011-02-01
  • 通讯作者: 沈 南, 电子信箱: nanshensibs@gmail.com/shennand@online.sh.cn。
  • 作者简介:范 薇（1985—）, 女, 硕士生；电子信箱: fanwei1202003@sina.com。
  • 基金资助:

    国家杰出青年科学基金项目（81025016）；国家高新技术发展计划 (“863”计划) (2007AA02Z123)；国家自然科学基金(30700734, 30801026, 30971632)；上海市自然科学基金(07ZR14130, 10ZR1434900)；上海市科委重点项目(08JC1414700, 10JC1409300)；上海市重点学科建设项目(T0203)；上海市医院临床科研资源共享平台项目(SHDC12007205)

Expression of miR-31 in peripheral blood of patients with systemic lupus erythematosus

FAN Wei1, TANG Yuan-jia2, QU Bo2, CUI Hui-juan2, HUANG Xin-fang1, CHEN Shun-le1, SHEN Nan1,2   

  1. 1.Department of Rheumatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Rheumatology, Shanghai 200001, China；2.Laboratory of Molecular Rheumatology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences/Institute of Health Sciences, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    National Outstanding Youth Science Foundation of China, 81025016；National High Technology Research and Development Program of China, 863 program, 2007AA02Z123；National Natural Science Foundation of China, 30700734, 30801026, 30971632；Shanghai Natural Science Foundation, 07ZR14130, 10ZR1434900；Key Program of Shanghai Science and Technology Committee, 08JC1414700, 10JC1409300；Shanghai Key Discipline Construction Project, T0203；Shanghai Clinical Research Resource Sharing Platform Project, SHDC12007205

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摘要：

目的 探讨系统性红斑狼疮（SLE）患者外周血细胞微小RNA （miR-31)的表达及其临床意义。方法 以36例SLE患者和23名健康对照者作为研究对象，采集外周静脉ACD抗凝血，Real-Time PCR检测miR-31表达。分析SLE患者疾病活动指数（SLEDAI）、肾脏受累程度指标（Renal-SLEDAI积分）及临床用药与外周血细胞miR-31表达的相关性。结果 SLE患者的外周血细胞miR-31表达显著低于健康对照者,差异有统计学意义（P<0.001）。SLE患者的外周血细胞miR-31表达与SLEDAI积分和Renal-SLEDAI积分呈显著负相关（r=-0.330，P=0.043； r=-0.337，P=0.044），与临床用药情况无显著相关性(P>0.05)。结论 SLE患者外周血细胞miR-31表达水平与疾病活动及肾脏损害程度相关，作为重要生物标志物有可能为SLE的早期诊断、治疗和预后评估提供一定的依据。

关键词: 系统性红斑狼疮, 微小RNA, miR-31

Abstract:

Objective To investigate the expression and clinical significance of microRNA (miR-31) in peripheral blood cells of patients with systemic lupus erythmatosus (SLE). Methods Thirty-six patients with SLE and 23 healthy controls were selected, peripheral ACD anti-coagulation blood was obtained, and the expression of miR-31 was detected by RealTime PCR. The relationship between the expression of miR-31 in peripheral blood cells and SLE disease activity index(SLEDAI), parameters of degree of renal injury (Renal-SLEDAI score) and clinical medication was analysed. Results The expression of miR-31 in peripheral blood cells of patients with SLE was significantly lower than that of healthy controls (P<0.001). The expression of miR-31 in peripheral blood cells of patients with SLE was negatively correlated with SLEDAI and Renal-SLEDAI score （r=-0.330，P=0.043；r=-0.337，P=0.044）, while no correlation was found between the expression of miR-31 and clinical medication (P<0.05). Conclusion The expression of miR-31 in peripheral blood cells of patients with SLE may be related to disease activity and degree of renal injury, and may serve as an important biomarker in the early diagnosis, treatment and prognosis assessment of SLE.

Key words: systemic lupus erythmatosus, microRNA, miR-31