上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (2): 187-.doi: 10.3969/j.issn.1674-8115.2011.02.015

• 论著（基础研究） • 上一篇    下一篇

白藜芦醇在诱导人胃癌HGC27细胞凋亡中的机制研究

杨宏梅, 邢 影, 郑美珍   

  1. 辽宁省肿瘤医院内科, 沈阳 110042
  • 出版日期:2011-02-28 发布日期:2011-03-01
  • 作者简介:杨宏梅（1971—）, 女, 副主任医师, 博士；电子信箱: ZJO5314784@163.com。

Mechanism of apoptosis induced by Resveratrol |in human gastric cancer HGC27 cells

YANG Hong-mei, XING Ying, ZHENG Mei-zhen   

  1. Department of Internal Medicine, Provincial Tumor Hospital of Liaoning, Shenyang 110042, China
  • Online:2011-02-28 Published:2011-03-01

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摘要：

目的 探讨白藜芦醇（Res）干预人胃癌HGC27细胞后，PTEN与p-Akt信号通路中，VEGF、MMP-9表达差异并探讨其相互关系。方法 应用体外HGC27细胞培养技术，采用CCK-8实验测定Res对HGC27细胞生长抑制率的影响；采用流式细胞术分析细胞周期；采用Western blotting检测Res对HGC27细胞中凋亡相关基因PTEN和p-Akt的影响；采用ELISA法检测Res干预前后HGC27细胞的VEGF和MMP-9蛋白分泌水平的变化。结果 Res对HGC27细胞的生长抑制率存在浓度和时间依赖性（P<0.05）；Res干预HGC27后48 h，细胞周期分析显示DNA合成期（S期）细胞比例明显减少，并呈现剂量相关性（r=－0.930，P=0.007）；PTEN相对表达量与Res干预浓度呈正相关（r=0.951，P=0.04）；p-Akt、VEGF和MMP-9相对表达量均与Res干预浓度呈负相关（r=－0.969，P=0.03；r=－0.894，P=0.02；r=－0.881，P=0.01）。结论 Res能够抑制人胃癌HGC27细胞的增殖，并且诱导其凋亡，其机制可能与上调PTEN的表达和抑制Akt的磷酸化有关，并通过调控VEGF和MMP9的分泌实现HGC27的增殖抑制及凋亡。

关键词: 白藜芦醇, HGC27细胞, 细胞凋亡, PTEN, p-Akt

Abstract:

Objective To investigate the relationship between the expression of VEGF and MMP-9 in PTEN and p-Akt signaling pathway of resveratrol affecting gastric cancer HGC27 cells. Methods The effects of resveratrol on growth inhibition rate and cell cycle of HGC27 cells were examined by CCK-8 test and flow cytometry, respectively. The effects of resveratrol on apoptosis-related PTEN and p-Akt gene in HGC27 cells were detected by Western blotting, and the contents of VEGF and MMP-9 protein in HGC27 cells before and after resveratrol treatment were determined by ELISA. Results The growth inhibition rate of HGC27 cells by resveratrol treatment was concentration- and time-dependent （P<0.05）. Forty-eight hours after resveratrol treatment, cell cycle analysis indicated that the percent of HGC27 cells with DNA synthesis (S phase) significantly decreased, which exhibited a negative correlation with dosage （r=－0.930, P=0.007）. There was a positive relationship between the relative expression of PTEN and Res concentration （r=0.951，P=0.04）, while there was a negative relationship between the relative expression of p-Akt, VEGF and MMP-9 and Res concentration （r=－0.969，P=0.03；r=－0.894，P=0.02；r=－0.881，P=0.01）. Conclusion Resveratrol could inhibit the proliferation and induce apoptosis of human gastric cancer HGC27 cells, which may be related to the expression up-regulation of PTEN and phosphorylation inhibition of Akt. Resveratrol could also regulate the expression of VEGF and MMP-9 to inhibit the proliferation and apoptosis of HGC27 cells.

Key words: resveratrol, HGC27 cell, apoptosis, PTEN, p-Akt