上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (4): 385-.doi: 10.3969/j.issn.1674-8115.2012.04.002

• 专题报道（排尿功能障碍及盆底重建） • 上一篇    下一篇

硫酸鱼精蛋白联合脂多糖构建间质性膀胱炎动物模型的研究

吕坚伟, 沙建军, 张连华, 冷 静, 薄隽杰, 刘东明, 黄翼然   

  1. 上海交通大学 医学院附属仁济医院泌尿外科 |上海交通大学尿失禁及盆底重建诊治中心, 上海 200001
  • 出版日期:2012-04-28 发布日期:2012-04-27
  • 通讯作者: 黄翼然, 电子信箱: huangyiran@126.com。
  • 作者简介:吕坚伟（1976—）, 男, 主治医师, 博士；电子信箱: ljwass@hotmail.com。
  • 基金资助:

    上海市卫生局基金（2009Y033)

Protamine sulfate plus lipopolysaccharide in establishment of animal model of interstitial cystitis

LV Jian-wei, SHA Jian-jun, ZHANG Lian-hua, LENG Jing, BO Juan-jie, LIU Dong-ming, HUANG Yi-ran   

  1. Department of Urology, Renji Hospital, Center of Urinary Incontinence and Pelvic Reconstruction, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China
  • Online:2012-04-28 Published:2012-04-27
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2009Y033

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摘要：

目的 探讨利用硫酸鱼精蛋白联合脂多糖构建间质性膀胱炎动物模型的可行性。方法 将30只雌性SD大鼠分为试验组、环磷酰胺组和对照组，每组各10只。试验组采用10 mg/mL硫酸鱼精蛋白加750 μg/mL 脂多糖经尿道膀胱灌注；环磷酰胺组给予100 mg/kg的环磷酰胺腹腔内注射；对照组采用磷酸缓冲液膀胱灌注。采用苏木精-伊红染色计数膀胱组织单核炎症细胞，特殊染色计数脱颗粒的肥大细胞；测定各组大鼠尿液中组胺的质量浓度。结果 试验组膀胱组织可见大量单核炎症细胞以及较多脱颗粒状态的肥大细胞浸润；环磷酰胺组可见较多单核炎症细胞，但只有少量脱颗粒状态的肥大细胞；对照组只有少量的炎症细胞和未脱颗粒的肥大细胞。试验组和环磷酰胺组每个视野单核炎症细胞计数分别为76.5±9.8和69.3±6.9，均明显高于对照组的16.5±9.8(P<0.05)；试验组、环磷酰胺组和对照组每个视野脱颗粒肥大细胞计数分别为8.4±3.1、1.2±1.6和0.7±0.3，试验组明显高于其他两组（P<0.05）；试验组、环磷酰胺组和对照组大鼠尿液中组胺的质量浓度分别为(58.5±10.3)、(11.9±2.4)和(8.9±3.2)pg/mL，试验组明显高于其他两组（P<0.05）。结论 10 mg/mL硫酸鱼精蛋白加750 μg/mL脂多糖经尿道膀胱灌注构建的间质性膀胱炎动物模型符合间质性膀胱炎的病理生理表现，建模方法简单、可靠。

关键词: 硫酸鱼精蛋白, 脂多糖, 间质性膀胱炎, 动物模型

Abstract:

Objective To investigate the feasibility of establishment of animal model of interstitial cystitis by protamine sulfate plus lipopolysaccharide. Methods Thirty female SD rats were divided into experiment group, cyclophosphamide group and control group, with 10 rats in each group. Transurethral bladder perfusion of 10 mg/mL protamine sulfate plus 750 μg/mL lipopolysaccharide was performed in experiment group, intraperitoneal injection of 100 mg/kg cyclophosphamide was conducted in cyclophosphamide group, and PBS solution perfusion was administered in control group. Mononuclear inflammatory cells in bladder tissues were counted with HE staining, degranulated mast cells were counted with special staining, and the urine mass concentrations of histamine in groups were measured. Results A large number of mononuclear inflammatory cells and degranulated mast cells in bladder tissues were observed in experiment group, a large number of mononuclear inflammatory cells and a small number of degranulated mast cells in bladder tissues were found in cyclophosphamide group, and a small number of mononuclear inflammatory cells and degranulated mast cells in bladder tissues were detected in control group. The numbers of mononuclear inflammatory cells per visual field in experiment group (76.5±9.8) and cyclophosphamide group (69.3±6.9) were significantly larger than that in control group (16.5±9.8)(P<0.05). The number of degranulated mast cells per visual field in experiment group (8.4±3.1) was significantly larger than those in cyclophosphamide group (1.2±1.6) and control group (0.7±0.3)(P<0.05). The urine mass concentration of histamine in experiment group [(58.5±10.3) pg/mL] was significantly higher than those in cyclophosphamide group [(11.9±2.4) pg/mL] and control group [(8.9±3.2) pg/mL](P<0.05). Conclusion The animal model of interstitial cystitis established by 10 mg/mL protamine sulfate plus 750 μg/mL lipopolysaccharide is in line with the pathophysiological characteristics of interstitial cystitis, and the way of establishment is simple and reliable.

Key words: protamine sulfate, lipopolysaccharide, interstitial cystitis, animal model