上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

高脂饮食对孕鼠代谢及胎盘炎症和氧化应激反应的影响

陈 轩1，李华萍2   

  1. 1.苏州大学医学院， 苏州 215000； 2.上海交通大学附属第六人民医院妇产科， 上海 200233
  • 出版日期:2013-08-28 发布日期:2013-09-16
  • 通讯作者: 李华萍, 电子信箱: hpli819@sohu.com。
  • 作者简介:陈 轩(1987—)， 女， 硕士生; 电子信箱: llf2206@163.com。
  • 基金资助:

    上海市科委自然科学基金（12ZR1422200）

Effects of high fat diet on metabolism and inflammatory and oxidative stress responses in placenta of pregnant mice

CHEN Xuan1, LI Hua-ping2   

  1. 1.Medical College of Soochow University, Suzhou 215000, China; 2.Department of  Obstetrics & Gynecology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2013-08-28 Published:2013-09-16
  • Supported by:

    Shanghai Science and Technology Committee Foundation，12ZR1422200

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摘要：

目的 观察孕前及孕期高脂饮食对孕鼠代谢及胎盘炎症和氧化应激反应的影响。方法 根据饲料的不同将40只SPF级C57BL/6J雌鼠随机分为高脂饮食组和对照组，喂养14周后与公鼠合笼，至孕18.5 d时处死孕鼠。观察两组孕鼠体质量、空腹血糖、胰岛素、血脂及血清炎症因子水平的变化。分离胎盘组织，检测炎症及氧化应激相关因子水平，包括低氧诱导因子1α(HIF-1α)、肿瘤坏死因子α （TNF-α）和白介素6 （IL-6）mRNA 的表达，丙二醛(MDA)含量，核因子κB（NF-κB）和磷酸化NF-κB (pNF-κB)的表达。结果 高脂饮食组孕鼠体质量、空腹血糖和胰岛素、血脂及血清IL-6、TNF-α水平均显著高于对照组(P<0.001)。与对照组比较，高脂饮食组胎盘组织中NF-κB和pNF-κB表达增强（P<0.001），pNF-κB/NF-κB升高（P<0.05），TNF-α、IL-6和HIF-1α mRNA表达上调（P<0.001），MDA含量增加（P<0.001）。结论 肥胖可致孕期代谢紊乱，并伴发胎盘炎症及氧化应激反应增强，可能影响血管内皮细胞功能状态，导致产科并发症的发生。

关键词: 高脂饮食, 肥胖, 胎盘, 缺氧, 氧化应激反应, 炎症反应

Abstract:

Objective To investigate the effects of high fat diet before and during pregnancy on metabolism and inflammatory and oxidative stress responses in placenta of pregnant mice. Methods Forty female C57BL/6J mice were randomly divided into high fat diet group and control group. After feeding for 14 weeks, female mice in two groups mated with male mice, and pregnant mice were sacrificed on gestational day 18.5. The body weight, fasting blood glucose and insulin, serum lipid and serum inflammatory factors of pregnant mice in two groups were observed. The placenta tissues were isolated, and the levels of inflammatory and oxidative stress related factors were determined, including the expression of hypoxia inducible factor-1α (HIF-1α) mRNA, tumor necrosis factor factor-α (TNF-α) mRNA and interleukin-6 (IL-6) mRNA, content of malondialdehyde (MDA) and expression of nuclear factor-κB (NF-κB) and phospho-NF-κB (pNF-κB). Results The body weight, fasting blood glucose and insulin, serum lipid and serum IL-6 and TNF-α in high fat diet group were significantly higher than those in control group (P<0.001). The expression of NF-κB and pNF-κB, pNF-κB /NF-κB, the expression of TNF-α, IL-6 and HIF-1α mRNA and the content of MDA in placenta tissues in high fat diet group were significantly higher than those in control group (P<0.001 or P<0.05). Conclusion Maternal obesity may aggravate metabolic disorders and alter placental inflammatory and oxidative stress responses, which may change the function of placental vascular endothelium and lead to the obstrical complications.

Key words: high fat diet, obesity, placenta, hypoxia, oxidative stress, inflammation response