上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (8): 894-.doi: 10.3969/j.issn.1674-8115.2018.08.007

• 论著·基础研究 • 上一篇    下一篇

采用磁共振弥散加权成像序列检测大脑中动脉闭塞模型大鼠的下丘脑梗死

吴依兰，李格飞，史妍慧，何欣威，赵颖，赵蓉 ，刘建仁   

  1. 上海交通大学医学院附属第九人民医院神经内科，上海 200011
  • 出版日期:2018-08-28 发布日期:2018-09-11
  • 通讯作者: 刘建仁，电子信箱：liujr021@vip.163.com。赵蓉，电子信箱：zr1703@126.com。为共同通信作者。
  • 作者简介:吴依兰（1992—），女，博士生；电子信箱： wuyilan0422@163.com。
  • 基金资助:
    上海市教育委员会高峰高原学科建设计划 (20161422)；上海市科学技术委员会生物医药重点项目（ 16411953100）；上海交通大学医学院临床研究项目 (DLY201614)

Detection of hypothalamic infarction in intraluminal middle cerebral artery occlusion model ratDWI scanning

WU Yi-lan, LI Ge-fei, SHI Yan-hui, HE Xin-wei, ZHAO Ying, ZHAO Rong, LIU Jian-ren   

  1. Department of Neurology, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2018-08-28 Published:2018-09-11
  • Supported by:
    Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20161422; Key Project of Biomedicine in Science and Technology Commission of Shanghai Municipality, 16411953100; Clinical Research Project of Shanghai Jiao Tong University School of Medicine, DLY201614

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摘要： 目的 ·检测并分析大脑中动脉闭塞（ middle cerebral artery occlusion，MCAO）模型大鼠的下丘脑梗死情况。方法 ·对 15只体质量 200～ 250 g、年龄 6～ 8周的 Sprague-Dawley大鼠采用线栓法建立短暂性 MCAO大鼠模型，梗死 90 min后，拔出栓线进行再灌注。采用磁共振弥散加权成像（ diffusion weighted MR imaging，DWI）序列活体检测 MCAO模型大鼠再灌注 1 h及 24 h下丘脑和大脑的梗死体积，分析下丘脑梗死体积与大脑梗死体积的关系；采用 2, 3, 5-氯化三苯基四氮唑（ 2, 3, 5-triphenyl tetrazolium chloride，TTC）染色检测大鼠再灌注 24 h下丘脑和大脑的梗死发生情况，并与再灌注 24 h DWI扫描检测的结果进行对比。结果 · 15只 Sprague-Dawley大鼠成功建立了 MCAO/再灌注模型。再灌注 24 h，DWI序列检测下丘脑梗死的发生比例为 100%，而 TTC染色显示下丘脑梗死的比例仅为 40%，表明 DWI扫描比 TTC染色更加敏感（ P0.001）。采用 DWI序列检测再灌注 1 h及 24 h下丘脑梗死的体积分别为 (8.59±2.89) mm3和 (11.65±3.19) mm3；下丘脑梗死体积与大脑梗死体积显著相关（ r0.573，P0.025），下丘脑梗死体积的变化也与大脑梗死体积的变化显著相关 (r0.554，P0.032)。结论 ·采用 DWI序列对短暂性 MCAO模型大鼠下丘脑梗死进行检测比采用 TTC染色更为敏感，且采用 DWI序列检测时发现下丘脑梗死体积与大脑梗死体积显著相关。

关键词: 中动脉闭塞, 再灌注模型, 下丘脑梗死, 磁共振弥散加权成像, 2, 3, 5-氯化三苯基四氮唑染色

Abstract:

Objective · To detect and evaluate the hypothalamic infarction in middle cerebral artery occlusion (MCAO) model rat. Methods · For 15 Sprague-Dawley rats weighed 200–250 g, aged 6–8 months, their right middle cerebral artery was occluded for 90 mina silicon-coated 4–0 nylon filament and reperfused. Sprague-Dawley rats underwent diffusion weighted MR imaging (DWI) scanning (at 1 h and 24 h after reperfusion) and 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining (at 24 h after reperfusion) to determine the hypothalamic and cerebral infarct volume. The relationship between hypothalamic infarct volume and cerebral infarction volume was analyzedDWI scanning. The results of TTC staining were compared with those of 24 h DWI scanning. Results · Fifteen Sprague-Dawley rats successfully received intraluminal MCAO/reperfusion procedures. The incidences of hypothalamic infarction on brain DWI scanning and TTC staining were 100% and 40% at 24 h after reperfusion, respectively. Therefore, DWI scanning was more sensitive than TTC staining to detect hypothalamic injury (P0.001). The hypothalamic infarct volume on DWI scanning was (8.59±2.89) mm3 and (11.65±3.19) mm3 at 1 h and 24 h after reperfusion, respectively. On DWI scanning, hypothalamic and cerebral infarct volume at 24 h after reperfusion were correlated with each other significantly (r0.573, P0.025), so were the increases of hypothalamic and cerebral infarct volume (r0.554, P0.032) 1 h to 24 h. Conclusion · DWI scanning was more sensitive thanTTC staining todetect hypothalamic injury in intraluminal transient MCAO model. Hypothalamic and cerebral infarct volume were correlated with each other.

Key words: middle cerebral artery occlusion (MCAO), reperfusion model, hypothalamic infarction, diffusion weighted MR imaging (DWI), 2,3, 5-triphenyl tetrazoliumchloride (TTC) staining

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