上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (10): 1148-.doi: 10.3969/j.issn.1674-8115.2019.10.008

• 论著·基础研究 • 上一篇    下一篇

SDF-1联合CGF应用于犬年轻恒牙牙髓样组织再生的研究

乔 璐1，王慧慧1，齐 颖2，付梦辰1，颜燕宏1，赵玉梅1   

  1. 1. 上海牙组织修复与再生工程技术中心，同济大学口腔医学院儿童口腔医学教研室，上海 200072；2.上海市嘉定区牙病防治所，上海 201800
  • 出版日期:2019-10-28 发布日期:2019-11-22
  • 通讯作者: 赵玉梅，电子信箱：yumeizhao@tongji.edu.cn。
  • 作者简介:乔 璐（1992—），女，住院医师，硕士；电子信箱：qiaolujoyce@163.com。
  • 基金资助:
    上海市科学技术委员会科研计划项目（14411963600）

Evaluation of SDF-1 complex with CGF applied to canine dental-pulp-like tissue regeneration

QIAO Lu1, WANG Hui-hui1, QI Ying2, FU Meng-chen1, YAN Yan-hong1, ZHAO Yu-mei1   

  1. 1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Pediatric Dentistry, School of Stomatology, Tongji University, Shanghai 200072, China; 2. Jiading District Odontopathy Dispensary, Shanghai 201800, China
  • Online:2019-10-28 Published:2019-11-22
  • Supported by:
    Project of Science and Technology Commission of Shanghai Municipality，14411963600

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摘要： 目的·探讨以浓缩生长因子（concentrated growth factor，CGF）为支架，联合基质细胞衍生因子1（stromal cell-derived factor-1，SDF-1）应用于年轻恒牙牙髓再生的可行性。方法·体外分离培养犬牙髓细胞（dental pulp cells，DPC），观察SDF-1和CGF对其增殖、分化的影响；建立比格犬年轻恒切牙无髓模型，分为天然牙髓组（A组）、空管组（B组）、填充CGF组（C组）和复合SDF-1/CGF组（D组），10周后行影像学检查、RT-PCR及组织学观察。结果·CGF浸提液（concentrated growth factor extraction，CGFe）促进DPC体外增殖，联合SDF-1可加强其促增殖作用，且可促进细胞成牙、成血管分化；影像学检查示4组根尖均有不同程度生长；RT-PCR示D组新生组织中成牙、成血管相关基因表达均高于C组；组织学观察示C组和D组产生新生牙本质及类牙髓样组织，而B组仅在根尖部产生类牙骨质。结论·CGF可作为年轻恒牙牙髓样组织再生的有效支架，与SDF-1联合应用可促进这一过程。

关键词: 年轻恒牙, 牙髓再生, 基质细胞衍生生长因子, 浓缩生长因子

Abstract:

Objective · To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor (CGF) as a scaffold and stromal cell-derived factor-1 (SDF-1). Methods · Canine dental pulp cells (DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle’s immature incisors which were divided into four groups: natural pulp (A), empty cannel (B), CGF-filling (C) and SDF-1/CGF-filling (D). After 10 weeks, specimens were checkedimaging examination, RT-PCR and histological observation. Results · CGF extraction (CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the s of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion · SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teethusing CGF as an effective scaffold.

Key words: immature permanent teeth, pulp regeneration, stromal cell-derived factor-1 (SDF-1), concentrated growth factor (CGF)

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