上海交通大学学报(医学版) ›› 2021, Vol. 41 ›› Issue (7): 858-864.doi: 10.3969/j.issn.1674-8115.2021.07.003

• 论著 · 基础研究 • 上一篇    

支链氨基酸分解代谢在肺癌细胞中的功能

贺艳琪1,2(), 迟锐1,2, 陈梦萍1,2, 陈思1,2, 刘春良1,2, 刘云霞1,2, 孙海鹏1,2()   

  1. 1.上海交通大学基础医学院病理生理学系,细胞分化与凋亡教育部重点实验室,上海 200025
    2.上海交通大学医学院附属同仁医院虹桥国际医学研究院,上海 200050
  • 出版日期:2021-07-28 发布日期:2021-08-03
  • 通讯作者: 孙海鹏 E-mail:jenniehyq@163.com;sun.haipeng@yahoo.com
  • 作者简介:贺艳琪(1996—),女,硕士生;电子信箱:jenniehyq@163.com
  • 基金资助:
    国家自然科学基金(81570717);上海市科技创新行动计划基础研究领域项目(16JC1404400)

Function of branched-chain amino acid catabolism in lung cancer cells

Yan-qi HE1,2(), Rui CHI1,2, Meng-ping CHEN1,2, Si CHEN1,2, Chun-liang LIU1,2, Yun-xia LIU1,2, Hai-peng SUN1,2()   

  1. 1.Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
    2.Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200050
  • Online:2021-07-28 Published:2021-08-03
  • Contact: Hai-peng SUN E-mail:jenniehyq@163.com;sun.haipeng@yahoo.com
  • Supported by:
    National Natural Science Foundation of China(81570717);Basic Research Projects of Shanghai Science and Technology Innovation Action Plan(16JC1404400)

摘要:

目的·探索支链氨基酸(branched-chain amino acid,BCAA)分解代谢在肺癌细胞中的功能和作用机制。方法·采用瞬时转染法分别向非小细胞肺癌细胞H1299、A549和HCC827转染含无义序列的小干扰RNA(small interfering negative control,siNC)、支链酮酸脱氢酶激酶(branched-chain keto acid dehydrogenase kinase,BCKDK)基因的小干扰RNA(small interfering BCKDK,siBCKDK),并采用蛋白质印迹法(Western blotting)检测siNC组和siBCKDK组中BCKDK表达水平、支链酮酸脱氢酶亚基E1α(branched-chain keto acid dehydrogenase e1,α polypeptide,BCKDE1α)及其磷酸化水平。采用CCK-8法检测上述2组细胞的增殖活力。用含0、100、200 μmol/L的BCKDK小分子抑制剂BT2(3,6-二氯-2-苯并噻吩羧酸)培养液分别培养上述3种细胞,采用Western blotting检测3种浓度BT2组细胞中BCKDE1α及其磷酸化水平。用CCK-8法检测0 μmol/L BT2组和200 μmol/L BT2组细胞的增殖活力。采用台盼蓝染色计算H1299和A549细胞中siNC组和siBCKDK组、0 μmol/L BT2组和200 μmol/L BT2组的细胞活率;并用碘化丙啶染色检测细胞周期,而后行Western blotting检测细胞周期依赖性蛋白激酶抑制因子1A(cyclin-dependent kinase inhibitor 1A,P21)的表达水平。结果·在H1299、A549、HCC827细胞中,与siNC组相比,siBCKDK组的BCKDK表达下调,BCKDE1α磷酸化水平下调,且细胞的增殖活力减弱(均P=0.000)。与0 μmol/L BT2组相比,100 μmol/L BT2组和200 μmol/L BT2组的上述3种细胞中BCKDE1α磷酸化水平下调,且200 μmol/L BT2组下调更明显;200 μmol/L BT2组较0 μmol/L BT2组的细胞增殖活力减弱(均P=0.000)。在H1299、A549细胞中,分别与siNC组、0 μmol/L BT2组相比,siBCKDK组、200 μmol/L BT2组的细胞活率均无差异,但发生了G0/G1期细胞周期阻滞(均P<0.05),且该2组的P21表达水平升高。结论·siBCKDK和BT2能够促进BCKDE1α去磷酸化,加快BCAA分解代谢,可能通过上调P21、阻滞细胞周期来抑制肺癌细胞的增殖。

关键词: 支链氨基酸, 肺癌, 细胞增殖

Abstract:

Objective·To explore the function and mechanism of branched-chain amino acid (BCAA) catabolism in lung cancer cells.

Methods·Small interfering negative control (siNC) and small interfering branched-chain keto acid dehydrogenase kinase (siBCKDK) were transfected into non-small cell lung cancer cells H1299, A549 and HCC827 by instantaneous transfection. The expression of BCKDK, branched-chain keto acid dehydrogenase e1, α polypeptide (BCKDE1α) and its phosphorylation in siNC group and siBCKDK group were detected by Western blotting. The proliferation activity of the above two groups of cells was detected by CCK-8 assay. The above three kinds of cells were cultured in culture medium containing 0, 100, 200 μmol/L BT2 (3, 6-dichlorobenzo[b]thiophene-2-carboxylic acid), respectively. The expression of BCKDE1α and its phosphorylation were detected by Western blotting. The proliferation activity of 0 μmol/L BT2 group and 200 μmol/L BT2 group was detected by CCK-8 assay. The cell viability of siNC group and siBCKDK group, 0 μmol/L BT2 group and 200 μmol/L BT2 group in H1299 and A549 cells was calculated by trypan blue staining. Cell number at different phases of cell cycle was detected by propidium iodide staining. Cyclin-dependent kinase inhibitor 1A (P21) expression was detected by Western blotting.

Results·In H1299, A549 and HCC827 cells, compared with siNC group, expression of BCKDK and phosphorylation of BCKDE1α in siBCKDK group were down-regulated, and the proliferation activity was decreased (all P=0.000). Compared with 0 μmol/L BT2 group, the phosphorylation of BCKDE1α was down-regulated in H1299, A549 and HCC827 cells in both 100 μmol/L BT2 group and 200 μmol/L BT2 group, but more obviously in 200 μmol/L BT2 group. The cell proliferation activity in 200 μmol/L BT2 group was decreased compared with that in 0 μmol/L BT2 group (all P=0.000). There was no significant difference in the cell viability of H1299 and A549 cells between siBCKDK group and siNC group, 200 μmol/L BT2 group and 0 μmol/L BT2 group, but G0/G1 cell cycle arrest occurred (all P<0.05) with the expression of P21 increasing.

Conclusion·siBCKDK and BT2 can promote the dephosphorylation of BCKDE1α and accelerate the catabolism of BCAA, which may inhibit the proliferation of lung cancer cells by up-regulating P21 and blocking cell cycle.

Key words: branched-chain amino acids (BCAA), lung cancer, cell proliferation

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