上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

1型和2型糖尿病大鼠肺组织脂联素受体的表达

郭竹英1，王世婷2，徐芒华2，高丰厚2   

  1. 1.上海交通大学 医学院附属第三人民医院 1.检验科， 2.实验中心， 上海 201900
  • 出版日期:2013-11-28 发布日期:2013-12-03
  • 作者简介:郭竹英(1967—)， 女， 副主任技师， 硕士， 硕士生导师; 电子信箱: zyguoo@126.com。
  • 基金资助:

    上海市宝山区科委项目（09-E-1）

Expressions of adiponectin receptors in lung tissue of type 1 and type 2 diabetic rats

GUO Zhu-ying1, WANG Shi-ting2, XU Mang-hua2, GAO Feng-hou2   

  1. 1.Clinical Laboratory, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China; 2.Experimental Center, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China
  • Online:2013-11-28 Published:2013-12-03
  • Supported by:

    Foundation of Science and Technology Commission of Baoshan District, Shanghai, 09-E-1

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摘要：

目的 观察1型糖尿病（1-DM）和2型糖尿病（2-DM）大鼠肺组织脂联素受体(AdipoR1和AdipoR2）的表达情况。方法 SD大鼠随机分为1-DM组（一次性腹腔内注射高剂量链脲佐菌素建模，普通饮食，8周后处死)、72 h组（一次性腹腔内注射高剂量链脲佐菌素建模，普通饮食，72 h后处死）、2-DM组（高脂饮食配合一次性腹腔内注射低剂量链脲佐菌素建模，8周后处死)和对照组（一次性腹腔内注射等容量枸橼酸缓冲液，普通饮食）。分别检测血清胰岛素和脂联素水平以及肺组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及AdipoR1和AdipoR2的表达，HE染色观察肺组织病理学改变。结果 与对照组比较，1-DM 组和2-DM组血清脂联素水平均显著降低(P<0.05)；2-DM组血清脂联素水平显著低于1-DM 组(P<0.05)；1-DM 组和2-DM组肺组织AdipoR1表达及SOD活性均显著低于对照组，而72 h组与对照组比较差异无统计学意义（P>0.05）；1-DM 组和2-DM组肺组织有基膜增厚、血管堵塞等病理学改变。结论 肺组织脂联素受体表达以AdipoR1为主，高血糖可引起肺损伤、肺AdipoR1表达及SOD活性降低。

关键词: 脂联素受体, 脂联素, 1型糖尿病, 2型糖尿病

Abstract:

Objective To investigate expressions of adiponectin receptors (AdipoR1, AdipoR2) in lung tissue in type 1 and type 2 diabetes mellitus rats induced by streptozotocin (STZ). Methods A total of 40 SD rats were randomly divided into 4 groups: control (Con) group, type 1 diabetes mellitus (1-DM) group, 72 h after STZ injection (72 h) group, and type 2 diabetes mellitus (2-DM) group. The rats in 1-DM group and 72 h group were injected STZ intraperitoneally with a dose of 60 mg/kg while the ones in Con group were only done with the same dose of citric acid buffer. The rats in 2-DM group were injected STZ intraperitoneally with a dose of 35 mg/kg. Serum insulin and adiponectin levels were evaluated by ELISA. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in lung tissue were measured. Expression of AdipoR1, AdipoR2 in lung tissue was determined by immunohistochemistry. The pathological changes of lung tissue were examined by HE staining under light microscope. Results The levels of serum adiponectin in 1-DM and 2-DM groups were lower than that in Con group (P<0.05). The level of serum adiponectin in 2-DM group was lower than that of 1-DM group (P<0.05). Compared with Con group, the expression of AdipoR1 and activity of SOD in lung tissue in 1-DM and 2-DM groups were lower while those in 72 h group were similar. In 1-DM and 2-DM groups, the rat lung tissue manifested injuries such as basement membrane thickening and blood vessel occlusion. Conclusion Adiponectin receptors expression in lung tissue is given priority to AdipoR1. High blood glucose may result in lung injury and decrease AdipoR1 expression and SOD activity.

Key words: adiponectin receptor, adiponectin, type 1 diabetes mellitus, type 2 diabetes mellitus