上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

姜黄素对多囊卵巢综合征模型大鼠血清和卵巢中VEGF表达的影响

王炼炼，李 聪，孟江萍，杨 曦   

  1. 重庆医科大学附属第一医院妇产科， 重庆 400016
  • 出版日期:2014-02-28 发布日期:2014-03-25
  • 作者简介:王炼炼（1976—）， 女， 主治医师， 硕士; 电子信箱: 343650062@qq.com。
  • 基金资助:

    国家自然基金青年资助项目（81100399）；重庆市科委资助项目（cstc2011jjA0111）

Effect of curcumin on expression of VEGF in polycystic ovary syndrome rat models

WANG Lian-lian, LI Cong, MENG Jiang-ping, YANG Xi   

  1. Department of Obstetrics and Gynecology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
  • Online:2014-02-28 Published:2014-03-25
  • Supported by:

    Young Scientists Project of National Natural Science Foundation of China, 81100399; Foundation of Chongqing Science and Technology Commission, cstc2011jjA0111

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摘要：

目的 观察姜黄素对多囊卵巢综合征（PCOS）模型大鼠血清和卵巢中血管内皮生长因子(VEGF)表达的影响。方法 选用42 d龄未成年雌性大鼠60只，采用脱氢表雄酮联合诺和灵N的方法构建大鼠PCOS的病理模型。将大鼠随机分为空白对照组、模型对照组、姜黄素低剂量组和姜黄素高剂量组。采用ELISA法检测各组大鼠血清中VEGF的表达水平；采用免疫组织化学法检测各组大鼠卵巢组织中VEGF的表达水平；Western blotting法检测VEGF的蛋白水平表达。结果 ELISA法结果显示，PCOS大鼠血清中的VEGF表达水平明显高于空白对照组，差异有统计学意义(P<0.01)；而给予姜黄素腹腔注射后，血清中的VEGF表达较模型对照组均有所降低，且高剂量组的表达低于低剂量组的表达（P<0.05）。免疫组织化学和Western blotting结果显示：VEGF蛋白在模型对照组卵巢中的表达阳性率均高于空白对照组，差异有统计学意义（P<0.01），而姜黄素干预后其表达水平均降低，且高剂量组的表达阳性率显著低于低剂量组（P<0.05）。结论 姜黄素能够显著降低PCOS大鼠血清和卵巢组织中VEGF的表达，提示其可能对PCOS具有一定的治疗作用。

关键词: 姜黄素, 多囊卵巢综合征, 血管内皮生长因子

Abstract:

Objective To explore the effect of curcumin on the expression of VEGF in the polycystic ovary syndrome (PCOS) rat models. Methods Sixty immature female rats of 42 d old were selected. The PCOS rat model was established by injecting dehydroepiandrosterone and insulin. The rats were randomly divided into four groups, namely normal control group, model control group, lose-dose curcumin group, and high-dose curcumin group. The serum VEGF expression was measured by enzyme linked immunosorbent assay (ELISA); the expression of VEGF in the ovarian tissues was detected by immunohistochemical SP method; and protein level of VEGF was measured by Western blot. Results The results of ELISA showed that the expression level of VEGF in the serum of PCOS rats from the model group was significantly higher than that of the normal group (P<0.01). After curcumin was intraabdominally given, the expressions of VEGF in low- and high-dose curcumin groups were lower than that in the model group, and the expression of VEGF in high-dose group was lower than that in lose dose group (P<0.05). The results of immunohistochemical SP method and Western blotting showed that in ovarian tissues, the expression of VEGF protein in the model group was significantly higher than that in the normal group (P<0.01). After curcumin was given, the expressions of VEGF in high- and low-dose curcumin groups decreased, and the expression of VEGF in high-dose group was significantly lower than that in low-dose group (P<0.05). Conclusion Curcumin can lower the expression of VEGF in ovarian tissues of PCOS rats, and it may have certain therapeutic effect on PCOS.

Key words: curcumin, polycystic ovary syndrome, vascular endothelial growth factor