上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

沉默CCL19对结直肠癌增殖、迁移、侵袭和促血管形成的影响

陆 军1,2，赵敬坤1,2，陆爱国1，冯 浩3，王蒲雄志1，张 卓1，宗雅萍1，马君俊1，刘炳亚2，郑民华1   

  1. 上海交通大学 医学院附属瑞金医院 1.微创外科， 2.上海市消化外科研究所， 上海 200025； 3.慕尼黑大学医学院， 德国 慕尼黑 80539
  • 出版日期:2014-05-28 发布日期:2014-05-30
  • 通讯作者: 陆爱国, 电子信箱: adams_lu66@yahoo.com.cn。
  • 作者简介:陆 军（1987—）， 男， 硕士生; 电子信箱: 13788989913@163.com。
  • 基金资助:

    国家自然科学基金（81201625）；上海市科委引导项目（124119a0900）

Effects of silencing CCL19 on migration, invasion, and proangiopoiesis of colorectal cancer cells

LU Jun1,2, ZHAO Jing-kun1,2, LU Ai-guo1, FENG Hao3, WANGPU Xiong-zhi1, ZHANG Zhuo1, ZONG Ya-ping1, MA Jun-jun1, LIU Bing-ya1, ZHENG Min-hua1   

  1. 1.Department of Minimally Invasion Surgery, 2.Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3.The University of Munich School of Medicine, Munich 80539, Germany
  • Online:2014-05-28 Published:2014-05-30
  • Supported by:

    National Natural Science Foundation of China, 81201625； Guidance Project of Science and Technology Commission of Shanghai Municipality，124119a0900

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摘要：

目的 研究趋化因子CCL19在结直肠肿瘤中的作用。方法 采用Real-Time PCR和Western blotting技术筛选高表达CCL19的细胞株SW620细胞作为研究对象，并用siRNA沉默SW620中的CCL19基因。分别采用细胞增殖实验、Transwell迁移、侵袭实验和小管形成实验来检测SW620细胞的增殖、迁移、侵袭和促血管形成能力的变化。结果 Western blotting和Real-Time PCR证实SW620中CCL19相对其他细胞株高表达。经siRNA-CCL19干扰后，SW620细胞在48~120 h内细胞增殖能力显著上升（P<0.05），细胞迁移、侵袭能力亦明显上升（P<0.05），促血管形成能力明显增强。结论 SW620是相对高表达CCL19的细胞株，沉默CCL19基因后可以明显促进SW620细胞在体外的增殖、迁移、侵袭和促血管形成能力，因此认为CCL19在结直肠癌发展中起着抑制作用，并有应用于抗癌药物研究的前景。

关键词: 趋化因子CCL19, 细胞增殖, 细胞迁移, 细胞侵袭, 血管形成

Abstract:

Objective To investigate the role of chemokine CCL19 in colorectal carcinoma tissues. Methods SW620 Cell line with highest expression level of CCL19 was screened for the study by the Real-Time quantitative PCR and Western blot. CCL19 of SW620 was silenced by siRNA. The changes of proliferation, migration and invasion, and proangiopoiesis abilities of SW620 were detected by the CCK-8, Transwell, and tube formation assay, respectively. Results The Western blotting and Real-Time PCR confirmed that the expression of CCL19 in SW620 was higher than that of other cell lines. The proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells within 48-120 h were significantly improved after CCL19 being silenced. Conclusion The expression level of CCL19 in SW620 is relatively high. Silencing CCL19 can significantly enhance the proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells in vitro, so it is believed that the CCL19 can inhibit the development of colorectal cancer and is potential to be applied to the research of anticancer drugs.

Key words: chemokine CCL19, cell proliferation, cell migration, cell invasion, vascularization