上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

子宫内膜异位症不孕与CD4+CD25+FoxP3调节性T淋巴细胞的相关性研究

梁 艳，陈淑芳，张 健   

  1. 上海交通大学 医学院附属国际和平妇幼保健院， 上海 200010
  • 出版日期:2014-07-28 发布日期:2014-08-11
  • 通讯作者: 张 健, 电子信箱: zjian65@hotmail.com。
  • 作者简介:梁 艳（1981—）， 女， 主治医师， 硕士; 电子信箱: ly547@126.com。
  • 基金资助:

    上海市卫生局青年科学研究项目（2010Y1）

Relationship between CD4+CD25+FoxP3 regulatory T cells and endometriosis associated infertility

LIANG Yan, CHEN Shu-fang, ZHANG Jian   

  1. International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200010, China
  • Online:2014-07-28 Published:2014-08-11
  • Supported by:

    Foundation for Young Scientists of Shanghai Municipal Health Bureau, 2010Y1

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摘要：

目的 探讨子宫内膜异位症（EM）不孕与围种植期外周血及在位内膜CD4+CD25+FoxP3调节性T淋巴细胞（Tregs）变化的相关性。方法 采用流式细胞术检测27例不同期别EM不孕患者外周血单个核细胞CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞的百分比，RT-PCR检测在位内膜CD4+CD25+FoxP3 mRNA表达水平。以已生育非EM女性作为对照组（n=20）。结果 与对照组比较，轻度EM不孕组外周血单个核细胞中CD4+CD25+FoxP3 Tregs数量显著增多，其占CD4+T淋巴细胞百分比增高，差异均有统计学意义（P<0.05）；重度EM不孕组CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞百分比均显著高于轻度EM不孕组，差异均有统计学意义（P<0.05）。在位内膜FoxP3 mRNA相对表达量检测结果显示，轻度EM不孕组显著高于对照组 (P=0.000 1)，重度EM组显著高于轻度EM组和对照组（P<0.000 1）。结论 EM不孕围种植期CD4+CD25+FoxP3 Tregs与内膜容受性异常导致不孕的机制无直接关系；CD4+CD25+FoxP3 Tregs可能与EM的发生和发展有关，可能加重EM所致病变而导致不孕。

关键词: 子宫内膜异位症, CD4+CD25+FoxP3, 不孕症

Abstract:

Objective To explore the relationship between the endometriosis (EM) associated infertility and changes of CD4+CD25+FoxP3 Tregs in the peripheral blood and eutopic endometrium during peri-implantation phase. Methods Twenty-seven women with EM associated infertility of different stages were selected. The CD4+CD25+FoxP3 Tregs counts in peripheral blood mononulear cells (PBMCs) and the ratio of CD4+T cells and PBMCs were detected by the flow cytometry. The expression of CD4+CD25+FoxP3 mRNA of eutopic endometrium was detected by the RT-PCR. The controls were twenty procreated women without EM (n=20). Results Compared to the controls, the CD4+CD25+FoxP3 Tregs counts in PBMCs and the ratio of CD4+ T cells and PBMCs of women with minor EM were significantly higher. The differences were statistically significant (P<0.05). The CD4+CD25+FoxP3 Tregs counts in PBMCs and the ratio of CD4+ T cells and PBMCs of women with severe EM were significantly higher than those of women with minor EM. The differences were statistically significant (P<0.05). The relative expression of FoxP3 mRNA of eutopic endometrium showed that the expression of women with minor EM was significantly higher than that of controls (P=0.000 1), and the expression of women with severe EM was significantly higher than that of controls and women with minor EM (P<0.000 1). Conclusion The CD4+CD25+FoxP3 Tregs in the peri-implantation phase are not directly relevant to the pathogenesis of abnormal endometrial receptivity of infertile women with EM. CD4+CD25+ FoxP3 Tregs may be relevant to the incidence and development of EM and may aggravate lesions caused by EM and lead to the infertility.

Key words: endometriosis, CD4+CD25+ FoxP3, infertility