上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

AMD3100对坐骨神经分支选择性损伤模型大鼠的影响

黄雪花1，刘宁洁2，戴丽华1，马柯1   

  1. 1.上海交通大学 医学院附属新华医院疼痛科， 上海200092； 2.江西南昌市洪都中医院药剂科， 南昌330008
  • 出版日期:2016-01-28 发布日期:2016-02-26
  • 通讯作者: 马柯, 电子信箱: marke72@163.com。
  • 作者简介:黄雪花(1971—)， 女， 苗族， 主治医师， 硕士; 电子信箱: 1035065207@qq.com。
  • 基金资助:

    国家自然科学基金（81371246）；上海市科学技术委员会基金（No.12ZR1419900）

Effects of AMD3100 on rats of spared nerve injury model

HUANG Xue-hua1, LIU Ning-jie2, DAI Li-hua1, MA Ke1   

  1. 1.Department of Pain, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China； 2.Department of Pharmacy， Hongdu Hospital of Traditional Chinese Medicine, Nanchang 330008， China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    National Natural Science Foundation of China, 81371246；Shanghai Science and Technology Commission Fund, 12ZR1419900。

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摘要：

目的 观察鞘内注射趋化因子受体CXCR4特异性拮抗剂AMD3100对坐骨神经分支选择性损伤（SNI）模型大鼠的影响。方法 雄性SD大鼠60只，随机分为3组，分别为AMD3100组（n=25）、对照组（n=25）和假手术组（n=10）。采用SNI术建立疼痛模型，AMD3100组鞘内连续注射AMD3100（1 μg/只，1次/d，持续14 d），对照组和假手术组同时鞘内注射等容积生理盐水。采用Von-Frey细丝测量大鼠患肢机械刺激缩足阈值(PWT)。分别于首次给药后14、21、28、35 d，采用Western blotting和免疫荧光法检测海马中胶质细胞原纤维酸性蛋白（GFAP）表达。结果 与假手术组相比，对照组大鼠PWT值术后明显降低，海马GFAP表达增高。鞘内连续注射AMD3100可显著提高大鼠PWT，下调海马GFAP表达。结论 鞘内注射AMD3100使SNI模型大鼠海马中GFAP表达下调，可有效缓解大鼠神经病理性疼痛的症状。

关键词: 神经病理性疼痛, AMD3100, 海马, 胶质细胞原纤维酸性蛋白

Abstract:

Objective To observe the effects of intrathecal injection of CXCR4 specific antagonist AMD3100 on rats of spared nerve injury (SNI) model. Methods A total of 60 male SD rats were randomly divided into 3 groups, i.e. sham group (n=10), control group (n=25), and AMD3100 group (n=25). SNI was adopted to establish the neuropathic pain model. Rats of AMD3100 group underwent continuous intrathecal injection of AMD3100 (1μg per mouse, once a day for two weeks), while rats of sham group and control group underwent intrathecal injection of the same volume of normal saline. The paw withdrawal threshold (PWT) under mechanical stimulus of affected extremities of rats was measured by Von-Frey filaments. The expression of glial fibrillary acidic protein (GFAP) in the hippocampus was detected by Western  blotting and immunofluorescence 14, 21, 28, and 35 d after first administration. Results Compared with sham group, PWT of control group significantly decreased after SNI operation and the expression of GFAP in the hippocampus increased. The continuous intrathecal injection of AMD3100 significantly increased the PWT of rats and down-regulated the expression of GFAP in the hippocampus. Conclusion The intrathecal injection of AMD3100 can down-regulate the expression of GFAP in the hippocampus of rats of SNI model and effectively alleviate symptoms of neuropathic pain of rats.

Key words: neuropathic pain, AMD3100, hippocampus, glial fibrillary acidic protein