上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

DLC1不同亚型对结肠癌细胞增殖及迁移的抑制作用

彭海霞1,2，侯明月3，褚以忞2，杨大明2，周锋利2，蒯榕2，徐莹2，侯照远4，#br# 李吉2，金震东1   

  1. 1.第二军医大学附属长海医院消化内科， 上海 200433； 2.上海交通大学  医学院附属同仁医院内窥镜室， 上海 200336； 3.山东省兰陵县人民医院， 兰陵 277799； 4.上海交通大学 医学院生物化学与分子细胞生物系， 上海 200025
  • 出版日期:2016-01-28 发布日期:2016-02-26
  • 通讯作者: 金震东, 电子信箱: zhendjin@126.com; 李吉, 电子信箱: lex365@sohu.com。
  • 作者简介:彭海霞（1971—）， 女， 主任医师， 博士生; 电子信箱: haixiapeng@163.com。
  • 基金资助:

    国家自然科学基金（81372028）；上海市卫生和计划生育委员会课题（201440426）；上海市第六人民医院医疗集团科研课题（ly201301）

Inhibitory effects of different isoforms of DLC1 on proliferation and migration of colon cancer cells

PENG Hai-xia1,2, HOU Ming-yue3, CHU Yi-min2, YANG Da-ming2, ZHOU Feng-li2, KUAI Rong2, #br# XU Ying2, HOU Zhao-yuan4, LI ji2, JIN Zhen-dong1   

  1. 1.Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2.Digestive Endoscopy Center, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; 4. Peoples Hospital of Lanling, Lanling 277799, China; 5.Department of Biochemistry & Molecular Cellular Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    National Natural Science Foundation of China, 81372028; Project of Shanghai Municipal Commission of Health and Family, 201440426; Scientific Research Project of Shanghai Sixth People's Hospital, ly201301。

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摘要：

目的 探讨肝癌缺失基因1（DLC1）不同亚型对结肠癌细胞系增殖及迁移的抑制作用。方法 通过qRT-PCR检测DLC1不同亚型在17对结肠癌组织与正常组织中的表达水平；同时过表达不同亚型的慢病毒感染结肠癌细胞SW1116，通过CCK8及Transwell实验观察不同亚型对结肠癌细胞株增殖及迁移的作用。结果 DLC1不同亚型在结肠癌组织中的表达均显著低于癌旁组织；过表达DLC1能够抑制结肠癌细胞系SW1116的增殖及迁移，其中DLC1亚型2 (DLC1-isoform2)的作用效果最强。结论 DLC1的不同亚型在结肠癌组织中的表达均低于癌旁组织，DLC1-isoform2对抑制结肠癌细胞的增殖及迁移作用最强。

关键词: 结肠癌, 肝癌缺失基因1, 亚型, 增殖, 迁移

Abstract:

Objective To investigate the inhibitory effect of different isoforms of deleted in liver cancer-1 (DLC1) on the proliferation and migration of the colon cancer cell line. Methods The expressions of isoforms of DLC1 in 17 colon cancer tissue samples and 17 normal tissue samples were detected by qRT-PCR. Colon cancer cells SW1116 were infected by over-expressed lentiviruses of different isoforms. The effects of different isoforms on the proliferation and migration of the colon cancer cell line were observed by CCK8 kit and Transwell test. Results The expressions of different isoforms of DLC1 in colon cancer tissues were significantly lower than those of adjacent normal tissues. Over-expression of DLC1 could inhibit the proliferation and migration of the colon cancer cell line SW1116 and the inhibitory effect of isoform2 (DLC1-isoform2) was the strongest. Conclusion The expressions of different isoforms of DLC1 in colon cancer tissues are significantly lower than those of adjacent normal tissues. The inhibitory effect of DLC1-isoform2 of DLC1 on the proliferation and migration of colon cancer cells is the strongest.

Key words: colon cancer, deleted in liver cancer-1, isoform, proliferation, migration