上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

脂联素对骨髓间充质干细胞增殖和凋亡的影响

付宗杰1，凌媛1，缪婕1，杨国源2，3，沈琳辉1   

  1. 上海交通大学 1.医学院附属瑞金医院老年病科， 上海 200025； 2.Med-X研究院神经生物和神经工程中心， 上海 200030； 3.医学院附属瑞金医院神经内科， 上海 200025
  • 出版日期:2016-05-28 发布日期:2016-05-26
  • 通讯作者: 沈琳辉, 电子信箱: linhui_shen@163.com； 杨国源, 电子信箱: gyyang0626@gmail.com。
  • 作者简介:付宗杰（1989—）， 女， 硕士生; 电子信箱: fu_zongjie@163.com。
  • 基金资助:

    国家自然科学基金（81300725）

Effects of adiponectin on the proliferation and apoptosis of mesenchymal stem cells

FU Zong-jie1, LIN Yuan1, MIAO Jie1, YANG Guo-yuan2,3, SHEN Lin-hui1   

  1. 1.Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Neuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China; 3.Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    National Natural Science Foundation of China， 81300725

PDF (PC)

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摘要：

目的 探讨脂联素（Apn）对骨髓间充质干细胞（MSCs）的增殖及凋亡的影响。方法 分离培养原代大鼠MSCs，用构建的小鼠Apn基因重组慢病毒（Lenti-Apn-IRES-EGFP）或对照空载病毒（Lenti-IRES-EGFP）转染MSCs，并测定转染率。转染后用CCK8试剂盒检测Apn-MSCs、Gfp-MSCs在体外的增殖情况，并在缺氧缺糖损伤模型（OGD）12 h后用流式细胞仪检测细胞凋亡率。结果 与对照组比较,Apn-MSCs细胞增殖速度更快（P=0.001）。在OGD 12 h后，Apn-MSCs凋亡率比对照组低（P=0.01）。结论 Apn可以促进MSCs的增殖，并减少缺氧、缺糖损伤后MSCs的凋亡。

关键词: 脂联素, 骨髓间充质干细胞, 细胞增殖, 细胞凋亡

Abstract:

Objective To explore the effects of adiponectin (Apn) on the proliferation and apoptosis of mesenchymal stem cells (MSCs). Methods Primary rat MSCs were separated and cultured. MSCs were transfected with constructed mouse Apn gene recombinant lentivirus (Lenti-Apn-IRES-EGFP）or control lentivirus (Lenti-IRES-EGFP) and the transfection efficiency was measured. The in vitro proliferation of Gfp-MSCs and Apn-MSCs were detected with the CCK8 kit after transfection. The apoptosis rate was measured with the flow cytometer 12 h after the construction of oxygen and glucose deprivation (OGD) model. Results The proliferation of Apn-MSCs was faster as compared with Gfp-MSCs (P＝0.001). The apoptosis rate of Apn-MSCs was lower than that of Gfp-MSCs 12 h after the construction of OGD model (P＝0.01). Conclusion Apn can promote the proliferation of MSCs and reduce the apoptosis of MSCs after oxygen and glucose deprivation.

Key words: adiponectin; , mesenchymal stem cells; , apoptosis; , proliferation