上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

不同强度体外冲击波联合骨髓间充质干细胞移植对大鼠骨缺损的修复效果

贾朗，黄荣忠，王愉乐，贾功伟   

  1. 重庆医科大学附属第二医院康复医学科，重庆 400010
  • 出版日期:2016-12-28 发布日期:2016-12-29
  • 通讯作者: 贾功伟，电子信箱：93413567@qq.com。
  • 作者简介:贾朗（1983—），男，主治医师，博士；电子信箱：93348047@qq.com。
  • 基金资助:

    重庆市卫计委医学科研项目（2012-2-064）

Effects of different intensity of extracorporeal shock wave combined with bone marrow mesenchymal stem cells transplantation on the repair of bone defect in rats

JIA Lang, HUANG Rong-zhong, WANG Yu-le, JIA Gong-wei   

  1. Department of Rehabilitation, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China
  • Online:2016-12-28 Published:2016-12-29
  • Supported by:

    Medical Scientific Research Projects Foundation of Chongqing, 2012-2-064

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摘要：

目的 ·观察不同强度体外冲击波结合骨髓间充质干细胞移植对大鼠骨缺损区修复的影响，探索该疗法中体外冲击波的最佳刺激参数。方法 · 150只SD大鼠随机分为5组，采用牙科钻在大鼠股骨中段钻一圆形骨缺损，建立骨缺损模型。体外分离、培养 SD大鼠骨髓间充质干细胞，将第3代骨髓间充质干细胞植入骨缺损处，给予不同刺激参数的冲击波干预。治疗后第2、4、6周各组处死10只大鼠，分别采用X线和H-E染色进行修复区放射学和组织学观察，评价各组放射学评分及组织学评分，并用Western blotting法检测相关成骨标志蛋白表达。结果 ·体外冲击波能显著促进骨缺损模型局部成骨活动。接受电压为8.5 kV、频率为2 Hz的体外冲击波干预的大鼠较其他组形成骨痂更早更多，组织学提示新生骨更成熟，蛋白印记表明成骨标志蛋白（Runx2、ALP、Col Ⅰ）表达升高最为显著。结论 ·体外冲击波联合局部骨髓间充质干细胞移植能促进局部成骨相关蛋白表达，加速骨缺损愈合。电压8.5 kV、频率2 Hz为本研究中冲击波最佳刺激参数。

关键词: 体外冲击波, 骨髓间充质干细胞, 骨缺损, 成骨分化

Abstract:

Objective · To observe the effects of different intensity of extracorporeal shock wave (ESW) combined with bone marrow mesenchymal stem cells (BMMSCs) transplantation on the repair of bone defect in rats and explore the optimal stimulation parameters of ESW in this therapy. Methods · A total of 150 SD rats were randomly divided into 5 groups. The bone defect model was built by drilling a round hole in the femur middle segment with a dental drill. BMMSCs of SD rats were isolated and cultivated in vitro. The third generation of BMMSCs was implanted into the bone defects where ESW with different stimulation parameters was applied. Ten rats in each group were sacrificed 2, 4, and 6 weeks after treatment. X-ray examination and HE staining were respectively used for radiological and histological observations. Radiological and histological scores were evaluated. Expressions of osteogenic related proteins were measured using Western blotting. Results · ESW significantly promoted the local osteogenic activity of the bone defect model. The rats receiving ESW of 8.5 kV and 2 Hz generated calluses earlier and more than other rats. Histological staining showed that their new bones were more mature and Western blotting indicated that the increase in expressions of osteogenic related proteins (RUNX2, ALP, and Col I) was more significant as well. Conclusion · ESW combined with BMMSCs transplantation can promote the expression of osteogenic related proteins and accelerate the healing of bone defect. 8.5 kV and 2 Hz are the optimal stimulation parameters of ESW for the repair of bone defect in this study.

Key words: extracorporeal shock wave, bone marrow mesenchymal stem cell, bone defect, osteogenic differentiation