上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (4): 369-.doi: 10.3969/j.issn.1674-8115.2018.04.003

• 论著·基础研究 • 上一篇    下一篇

系统性红斑狼疮患者CD4+CD25+CD45RA- T细胞中转录因子Bach2的表达及其影响

陆一闻 1, 2，郑英霞 2，潘秀军 2，聂红 1   

  1. 1. 上海交通大学基础医学院免疫学与微生物学系，上海 200025；2.上海交通大学医学院附属新华医院检验科，上海 200092
  • 出版日期:2018-04-28 发布日期:2018-05-03
  • 通讯作者: 聂红，电子信箱：hnie0823@126.com。
  • 作者简介:陆一闻（1987—），男，硕士生；电子信箱：luyiwengood@hotmail.com。
  • 基金资助:
    国家自然科学基金面上项目（81571525，81671590）

Expression of transcription factor Bach2 in CD4+CD25+CD45RA-T cells in patients with systemic lupus erythematosus and its effect

LU Yi-wen1, 2, ZHENG Ying-xia2, PAN Xiu-jun2, NIE Hong1   

  1. 1. Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025; 2. Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092
  • Online:2018-04-28 Published:2018-05-03
  • Supported by:
    National Natural Science Foundation of China, 81571525, 81671590

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摘要： 目的·研究系统性红斑狼疮（systemic lupus erythematosus，SLE）患者CD4+CD25+CD45RA-T细胞中转录因子BTB与CNC同源基因2（BTB and CNC homology 2, Bach2）的表达及其对细胞功能的影响。方法·采用流式细胞仪分选获得活动期SLE患者（SLE组）和健康对照者（对照组）外周血CD4+CD25+CD45RA-T细胞，荧光定量PCR和Western blotting检测该类细胞中Bach2的表达；分析SLE患者该类细胞中Bach2的平均荧光强度（median fluorescence intensity，MFI）与SLE疾病活动指数（systemic lupus erythematosus disease activity index，SLEDAI）的相关性。采用流式细胞术比较SLE患者IL-17+CD4+CD25+CD45RA-T细胞和IL-17CD4+CD25+CD45RA-T细胞中Bach2的MFI。在Bach2过表达体系中，采用流式细胞术检测CD4+CD25+CD45RA-T细胞表达IL-17的细胞比例，采用ELISA方法检测细胞培养上清液中IL-17的含量。结果·与对照组相比，SLE组CD4+CD25+CD45RA-T细胞Bach2的mRNA和蛋白表达水平均显著降低（均P<0.01）；SLE组Bach2的MFI与SLEDAI呈显著负相关（R20.433，P0.001）。SLE患者IL-17+CD4+CD25+CD45RA-T细胞Bach2的MFI显著低于IL-17-CD4+CD25+CD45RA-T细胞（P0.013）。当Bach2过表达时，SLE患者CD4+CD25+CD45RA-T细胞表达炎症因子IL-17的细胞比例明显下降（P0.032），细胞培养上清液中IL-17含量显著降低（P0.008）。结论· SLE患者CD4+CD25+CD45RA-T细胞Bach2表达下降，在该类细胞中过表达Bach2可使IL-17表达下降。

关键词: 系统性红斑狼疮, CD4+CD25+CD45RA-T细胞, BTB与CNC同源基因2, 白细胞介素17

Abstract:

Objective · To study the of transcription factor BTB and CNC homology 2 (Bach2) in CD4+CD25+CD45RA-T cells patients with systemic lupus erythematosus (SLE) and its effect on cell function. Methods · The CD4+CD25+CD45RA-T cells active SLE patients and healthy volunteers were sortedflow cytometry. The of Bach2 in CD4+CD25+CD45RA-T cells was detectedfluorescence quantitative PCR and Western blotting. The correlation between the median flourscence indensity (MFI) of Bach2 in CD4+CD25+CD45RA- T cells and the disease activity index of SLE (SLEDAI) was analyzed. The MFI of Bach2 in IL-17+CD4+CD25+CD45RA-T cells was compared with that inIL-17-CD4+CD25+CD45RA-T cellsflow cytometry. In Bach2 over system, the of IL-17 in CD4+CD25+CD45RA- T cells was detectedflow cytometry and the concentration of IL-17 in the culture supernants was detectedELISA. Results · The mRNA and protein s of Bach2 in CD4+CD25+CD45RA-T cells SLE patients were significantly lower than those in healthy controls (P<0.01). There was a significant negative correlation between the MFI of Bach2 and SLEDAI (R20.433, P0.001) in patients with SLE. The of Bach2 in IL17+CD4+CD25+CD45RA-T cells was significantly lower than that in IL-17-CD4+CD25+CD45RA-T cells (P0.013). When Bach2 was overexpressed, the percentage of CD4+CD25+CD45RA-T cells SLE patients expressing inflammatory factor IL-17 decreased significantly (P0.032) and the IL-17concentration in cell culture supernatants markedly decreased (P0.008). Conclusion · The of Bach2 in CD4+CD25+CD45RA-T cells SLE patients decreases, and over of Bach2 in the cells leads to the falling of IL-17.

Key words: systemic lupus erythematosus, CD4+CD25+CD45RA-T cell, BTB and CNC homology 2 (Bach2), interleukin-17

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