上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (8): 874-.doi: 10.3969/j.issn.1674-8115.2018.08.004

• 论著·基础研究 • 上一篇    下一篇

孕前和孕期维生素 D缺乏对子代大鼠肠道菌群及抗菌肽 cathelicidin的影响

王磊 1，焦先婷 1，魏真真 1，刘斌 3，刘晓艳 3，余晓丹 1, 2   

  1. 1.上海交通大学医学院附属新华医院，环境与儿童健康教育部和上海市重点实验室，上海 200092；2.上海交通大学医学院附属上海儿童医学中心儿童保健科，上海 200127；3.福建农林大学食品科学学院，福州 350002
  • 出版日期:2018-08-28 发布日期:2018-09-11
  • 通讯作者: 余晓丹，电子信箱：xd_yu2003@126.com。
  • 作者简介:王磊（1992—），女，硕士生；电子信箱： 15961061113@163.com。
  • 基金资助:
    国家自然科学基金（ 81773411，81373004）；国家重点研发计划（ 2016YFC1305204）；上海市教育委员会高峰高原学科建设计划（ 20152220）

Effects of maternal vitamin D deficiency before and during pregnancy on intestinal flora and cathelicidin antimicrobial peptide in offspring rats

WANG Lei1, JIAO Xian-ting1, WEI Zhen-zhen1, LIU Bin3, LIU Xiao-yan3, YU Xiao-dan1, 2   

  1. 1. MOE-Shanghai Key Lab of Children′s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2. Department of Developmental and Behavioral Pediatrics, Shanghai Children′s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2018-08-28 Published:2018-09-11
  • Supported by:
    National Natural Science Foundation of China, 81773411, 81373004; National Key Research and Development Program of China, 2016YFC1305204; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20152220

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摘要： 目的 ·探讨孕前和孕期维生素 D缺乏对子代大鼠肠道菌群及抗菌肽 cathelicidin（cathelicidin antimicrobial peptide，CAMP）的影响。方法 ·将 24只 8周龄雌性 SD大鼠分为 3组，对照组（ C组）、维生素 D缺乏组（ VDD组）及维生素 D补充组（ VDS组）各 8只。通过特殊饲料饮食构建大鼠孕前和孕期维生素 D缺乏模型。母鼠孕第 14日用液相色谱 -串联质谱联用法检测血清 25(OH)D水平。子鼠 4周龄时，取血检测 25(OH)D水平；取粪便检测肠道菌群；取结肠组织，用实时荧光定量 RT-qPCR法、Western blotting法分别测定大鼠 CAMP的 mRNA及蛋白表达水平。结果 ·大鼠孕前和孕期维生素 D缺乏模型构建成功。 C组、VDD组及 VDS组子鼠肠道乳酸杆菌的相对丰度分别为 0.050±0.016、0.028±0.013及 0.033±0.021。与 C组相比， VDD组子鼠肠道乳酸杆菌的相对丰度显著降低（ P<0.05），结肠 CAMP的 mRNA及蛋白表达水平亦显著降低（均 P<0.05）。与 VDD组相比， VDS组子鼠肠道乳酸杆菌的相对丰度有升高的趋势，但差异无统计学意义；结肠 CAMP的 mRNA表达水平无明显改变，蛋白表达水平显著升高（ P<0.05）。结论 ·孕期维生素 D缺乏可致子代大鼠结肠 CAMP的 mRNA及蛋白表达水平和肠道乳酸杆菌的相对丰度显著降低。孕期维生素 D补充后，子代大鼠结肠 CAMP的蛋白表达水平显著升高，肠道乳酸杆菌的丰度也有升高的趋势。

关键词: 孕期, 维生素 D, 肠道菌群, 抗菌肽 cathelicidin

Abstract:

Objective · To explore the effects of maternal vitamin D deficiency before and during pregnancy on the intestinal flora and cathelicidin antimicrobial peptide (CAMP) in offspring rats. Methods · Twenty-four female Sprague-Dawley rats (8-week-old) were randomly assigned to three groups (n8 per group), i.e. control group (C group), vitamin D deficiency group (VDD group), and vitamin D supplement group (VDS group). Special diets were used to build the rat models of vitamin D deficiency before and during pregnancy. Maternal 25(OH)D level was detectedliquid chromatography tandem mass spectrometry (LC-MS/MS) at gestational day 14.At the age of 4 weeks in offsprings, the level of 25(OH)D was measured; the faeces were collected for the detection of intestinal flora; the mRNA and protein of CAMP were evaluatedreal-time quantitative RTPCR and Western blotting, respectively. Results · The rat models of vitamin D deficiency before and during pregnancy were successfully established.The relative abundance of intestinal Lactobacillus in theoffspring rats in C group,VDD group andVDS group was 0.050±0.016,0.028±0.013 and0.033±0.021, respectively. Compared with C group, the relative abundance of intestinal Lactobacillus in VDD group significantly decreased (P<0.05)，and the mRNA and protein of colonic CAMPsignificantly decreased as well (P<0.05). Compared with VDD group, VDS group showed a trend of increase in the abundance of intestinal Lactobacillus;themRNA ofcolonic CAMPdidn’tchange significantly, but the proteinof colonic CAMP significantly increased (P<0.05). Conclusion · Vitamin D deficiency during pregnancy results in decreased abundance of intestinal Lactobacilli as well as reduced mRNAand protein of colonic CAMPin offspring rats. Vitamin D supplementation significantly improves the protein of colonic CAMP and induces a trend of increase in the abundance of intestinal Lactobacilli in offspring rats.

Key words: pregnancy, vitamin D, intestinal flora, cathelicidin antimicrobial peptide(CAMP)

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